A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis

被引:1
作者
da Costa Capizzani, Carolina Paulino [1 ]
Cacador, Natalia Candido [1 ]
Marques, Elizabeth Andrade [2 ]
Levy, Carlos Emilio [3 ]
Tonani, Ludmilla [1 ]
Monteiro Marin Torres, Lidia Alice Gomes [4 ]
da Costa Darini, Ana Lucia [1 ]
机构
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP, Brazil
[2] Univ Estado Rio De Janeiro, Fac Ciencias Med, Dept Microbiol Imunol & Parasitol, Rio De Janeiro, RJ, Brazil
[3] Univ Estadual Campinas, Dept Patol Clin, Fac Ciencias Med, Campinas, SP, Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Serv Referencia Fibrose Cist, Dept Puericultura & Pediat,Hosp Clin, Ribeirao Preto, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Cystic fibrosis; Nonfermenting; Gram-negative; bacteria Identification scheme; Diagnosis; BURKHOLDERIA-CEPACIA COMPLEX; DIRECTED PCR; SP-NOV; BACILLI; EPIDEMIOLOGY; XYLOSOXIDANS; INFECTION; SAMPLES; SPUTUM; MS;
D O I
10.1016/j.bjm.2017.07.002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Identification of nonfermenting Gram-negative bacteria (NFGNB) of cystic fibrosis patients is hard and misidentification could affect clinical outcome. This study aimed to propose a scheme using polymerase chain reaction to identify NFGNB. This scheme leads to reliable identification within 3 days in an economically viable manner when compared to other methods. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
引用
收藏
页码:422 / 428
页数:7
相关论文
共 32 条
[1]   Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry for species identification of Nonfermenting Gram-Negative Bacilli [J].
Almuzara, Marisa ;
Barberis, Claudia ;
Traglia, German ;
Famiglietti, Angela ;
Soledad Ramirez, Maria ;
Vay, Carlos .
JOURNAL OF MICROBIOLOGICAL METHODS, 2015, 112 :24-27
[2]   Coexistence of Burkholderia, Cupriavidus, and Rhizobium sp nodule bacteria on two Mimosa spp. in Costa Rica [J].
Barrett, CF ;
Parker, MA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (02) :1198-1206
[3]   Detection and accurate identification of new or emerging bacteria in cystic fibrosis patients [J].
Bittar, F. ;
Rolain, J. -M. .
CLINICAL MICROBIOLOGY AND INFECTION, 2010, 16 (07) :809-820
[4]   Comparison of Traditional Phenotypic Identification Methods with Partial 5′ 16S rRNA Gene Sequencing for Species-Level Identification of Nonfermenting Gram-Negative Bacilli [J].
Cloud, Joann L. ;
Harmsen, Dag ;
Iwen, Peter C. ;
Dunn, James J. ;
Hall, Gerri ;
LaSala, Paul Rocco ;
Hoggan, Karen ;
Wilson, Deborah ;
Woods, Gail L. ;
Mellmann, Alexander .
JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (04) :1442-1444
[5]   Use of PCR analyses to define the distribution of Ralstonia species recovered from patients with cystic fibrosis [J].
Coenye, T ;
Spilker, T ;
Reik, R ;
Vandamme, P ;
LiPuma, JJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (07) :3463-3466
[6]   Burkholderia ambifaria sp nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates [J].
Coenye, T ;
Mahenthiralingam, E ;
Henry, D ;
LiPuma, JJ ;
Laevens, S ;
Gillis, M ;
Speert, DP ;
Vandamme, P .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1481-1490
[7]   Identification of Pandoraea species by 16S ribosomal DNA-based PCR assays [J].
Coenye, T ;
Liu, LX ;
Vandamme, P ;
LiPuma, JJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (12) :4452-4455
[8]   The complete genome, comparative and functional analysis of Stenotrophomonas maltophilia reveals an organism heavily shielded by drug resistance determinants [J].
Crossman, Lisa C. ;
Gould, Virginia C. ;
Dow, J. Maxwell ;
Vernikos, Georgios S. ;
Okazaki, Aki ;
Sebaihia, Mohammed ;
Saunders, David ;
Arrowsmith, Claire ;
Carver, Tim ;
Peters, Nicholas ;
Adlem, Ellen ;
Kerhornou, Arnaud ;
Lord, Angela ;
Murphy, Lee ;
Seeger, Katharine ;
Squares, Robert ;
Rutter, Simon ;
Quail, Michael A. ;
Rajandream, Mari-Adele ;
Harris, David ;
Churcher, Carol ;
Bentley, Stephen D. ;
Parkhill, Julian ;
Thomson, Nicholas R. ;
Avison, Matthew B. .
GENOME BIOLOGY, 2008, 9 (04)
[9]   Microbiological diagnostic procedures for respiratory cystic fibrosis samples in Spain: towards standard of care practices [J].
de Dios Caballero, Juan ;
del Campo, Rosa ;
Tato, Marta ;
de la Pedrosa, Elia Gomez G. ;
Cobo, Marta ;
Lopez-Causape, Carla ;
Gomez-Mampaso, Enrique ;
Oliver, Antonio ;
Canton, Rafael .
BMC MICROBIOLOGY, 2014, 14
[10]   Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center [J].
Ferroni, A ;
Sermet-Gaudelus, I ;
Abachin, E ;
Quesne, G ;
Lenoir, G ;
Berche, P ;
Gaillard, JL .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (10) :3793-3797