The Interaction of Klebsiella pneumoniae With Lipid Rafts-Associated Cholesterol Increases Macrophage-Mediated Phagocytosis Due to Down Regulation of the Capsule Polysaccharide

被引：19
作者：
Ares, Miguel A. [1 ]
Sansabas, Alejandro [1 ,2 ]
Rodriguez-Valverde, Diana [1 ,2 ]
Siqueiros-Cendon, Tania [2 ]
Rascon-Cruz, Quintin [2 ]
Rosales-Reyes, Roberto [3 ]
Dolores Jarillo-Quijada, Ma. [3 ]
Alcantar-Curiel, Maria D. [3 ]
Cedillo, Maria L. [4 ]
Torres, Javier [1 ]
Giron, Jorge A. [4 ]
De la Cruz, Miguel A. [1 ]
机构：
[1] Inst Mexicano Seguro Social, Unidad Invest Medica Enfermedades Infecciosas & P, Ctr Med Nacl Siglo XXI, Hosp Pediat, Mexico City, DF, Mexico
[2] Univ Autonoma Chihuahua, Fac Ciencias Quim, Chihuahua, Mexico
[3] Univ Nacl Autonoma Mexico, Fac Med, Unidad Med Expt, Mexico City, DF, Mexico
[4] Benemerita Univ Autonoma Puebla, Ctr Detecc Biomol, Puebla, Mexico
来源：
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2019年 / 9卷
关键词：
Klebsiella pneumoniae; cholesterol; capsule; phagocytosis; H-NS; RcsA; ESCHERICHIA-COLI; EXPRESSION; GENES; TRANSLOCATION; EPIDEMIOLOGY; PATHOGENESIS; INFECTION; VIRULENCE; PCR;
D O I：
10.3389/fcimb.2019.00255
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Klebsiella pneumoniae successfully colonizes host tissues by recognizing and interacting with cholesterol present on membrane-associated lipid rafts. In this study, we evaluated the role of cholesterol in the expression of capsule polysaccharide genes of K. pneumoniae and its implication in resistance to phagocytosis. Our data revealed that exogenous cholesterol added to K. pneumoniae increases macrophage-mediated phagocytosis. To explain this event, the expression of capsular galF, wzi, and manC genes was determined in the presence of cholesterol. Down-regulation of these capsular genes occurred leading to increased susceptibility to phagocytosis by macrophages. In contrast, depletion of cholesterol from macrophage membranes led to enhanced expression of galF, wzi, and manC genes and to capsule production resulting in resistance to macrophage-mediated phagocytosis. Cholesterol-mediated repression of capsular genes was dependent on the RcsA and H-NS global regulators. Finally, cholesterol also down-regulated the expression of genes responsible for LPS core oligosaccharides production and OMPs. Our results suggest that cholesterol plays an important role for the host by reducing the anti-phagocytic properties of the K. pneumoniae capsule facilitating bacterial engulfment by macrophages during the bacteria-eukaryotic cell interaction mediated by lipid rafts.
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页数：10
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