Overexpression of the integrin β1A subunit and the β1A cytoplasmic domain modifies the β-adrenergic regulation of the cardiac L-type Ca2+ current

被引:18
|
作者
Cheng, Q
Ross, RS
Walsh, KB [1 ]
机构
[1] Univ S Carolina, Sch Med, Dept Pharmacol Physiol & Neurosci, Columbia, SC 29208 USA
[2] VA Healthcare, Cardiol Sect, Dept Med, La Jolla, CA 92161 USA
[3] Univ Calif San Diego, San Diego, CA 92161 USA
关键词
integrins; Ca2+ channels; myocytes; beta-adrenergic; protein kinase A; BayK8644;
D O I
10.1016/j.yjmcc.2004.03.006
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Integrins are a family of cell-surface receptors that link the extracellular matrix (ECM) to the cellular cytoskeleton. The goal of this study was to determine the importance of the integrin beta(1) subunit in regulating cardiac L-type Ca2+ channel function. Neonatal rat ventricular myocytes were cultured on collagen membranes and infected with adenovirus expressing either the human beta(1A) integrin (Adbeta(1A)) or a chimeric protein consisting of the cytoplasmic tail domain of the beta(1A) integrin and the extracellular/transmembrane domain of the interleukin-2 receptor (AdTAC-beta(1)). Expression of the free beta(1) integrin tail (TAC-beta(1)), but not the full-length beta(1A) integrin, altered cell morphology and disrupted normal cell adhesion. When compared with myocytes infected with control virus, neither Acbeta(1A) nor AdTAC-beta(1) infection produced any significant change in the current vs. voltage relationship of the whole-cell Ca2+ current (I-Ca) or the kinetics of I-Ca decay. Expression of TAC-beta(1), but not beta(1A), induced a negative shift in the Ca2+ channel steady-state inactivation curve. Application of the beta-adrenergic receptor agonist isoproterenol produced over a 90% increase in I-Ca in control cells, but caused only an 18% increase in myocytes overexpressing the full-length beta(1A) integrin. In addition, beta-adrenergic stimulation resulted in a 5-10-fold increase in intracellular cAMP levels in control cells, but produced no significant response in Adbeta(1A)-infected cells. In contrast, expression of TAC-beta(1) was associated with an augmentation in the Ca2+ channel response to isoproterenol (160% increase) and the Ca2+ channel agonist BayK8644. Thus, integrin/ECM interactions may be critical in the regulation of I-Ca. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:809 / 819
页数:11
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