Weight adjustment in calculating surface ligand coverage or density for chromatographic bonded phases

被引：7

作者：

Li, TY ^{[1
]}

机构：

[1] Mississippi State Univ, Dept Chem, Mississippi State, MS 39762 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1035卷 / 01期

关键词：

weight adjustment; surface coverage; ligand density; stationary phase; end-capping;

D O I：

10.1016/j.chroma.2004.02.045

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method based on successive approximation is proposed for the calculation of surface coverage or ligand density of chemically bonded stationary phases from elemental analysis data. The approach could be used to calculate surface coverage in many stationary phase preparations, including end-capping and stepwise couplings. Key in this procedure is the use of successive approximation to simplify the adjustment of weight changes in stationary phase preparation. The method does not involve complicated mathematic equations. (C) 2004 Published by Elsevier B.V.

引用

页码：151 / 152

页数：2

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