Qualitative Analysis To Ascertain Genotypic Identity of or Differences between Mycobacterium tuberculosis Isolates in Laboratories with Limited Resources

被引：3
作者：
Sislema-Egas, Fernanda [1 ]
Jesus Ruiz-Serrano, Maria [1 ,2 ]
Bouza, Emilio [1 ,2 ,3 ]
Garcia-de-Viedma, Dario [1 ,2 ,4 ]
机构：
[1] Hosp Gen Univ Gregorio Maranon, Inst Invest Sanitaria Gregorio Maranon, Serv Microbiol & Enfermedades Infecciosas, Madrid, Spain
[2] CIBER Enfermedades Resp CIBERES06 06 0058, Madrid, Spain
[3] Univ Complutense Madrid, Fac Med, Dept Med, Madrid, Spain
[4] UCM UPM, CEI Campus Moncloa, Madrid, Spain
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 12期
关键词：
VARIABLE-NUMBER; MOLECULAR EPIDEMIOLOGY; CROSS-CONTAMINATION; SYSTEM; STRAIN;
D O I：
10.1128/JCM.02183-13
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Mycobacterium tuberculosis is currently genotyped using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing, although the high cost of this technique restricts its implementation in resource-limited settings. We designed a MIRU-VNTR format, MLP3 (MIRU-VNTR length polymorphism triplex), that is based on the qualitative comparison of 5 nonfluorescent 3-band fingerprints in conventional electrophoresis and minimizes costs and technical demands. MLP3 successfully resolved cross-contamination alerts, discriminated reinfections from reactivations, clarified suspected microepidemics, and tracked transmission events of high epidemiological interest.
引用
收藏
页码：4230 / 4233
页数：4
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