Protein Secretion in Gram-Positive Bacteria: From Multiple Pathways to Biotechnology

被引:51
作者
Anne, Jozef [1 ]
Economou, Anastassios [1 ]
Bernaerts, Kristel [2 ]
机构
[1] Katholieke Univ Leuven, Lab Mol Bacteriol, Dept Microbiol & Immunol, Rega Inst Med Res, B-3000 Leuven, Belgium
[2] Katholieke Univ Leuven, Dept Chem Engn, Bio & Chem Syst Technol Reactor Engn & Safety Sec, Celestijnenlaan 200F, B-3001 Leuven, Belgium
来源
PROTEIN AND SUGAR EXPORT AND ASSEMBLY IN GRAM-POSITIVE BACTERIA | 2017年 / 404卷
关键词
ARGININE TRANSLOCATION PATHWAY; I SIGNAL PEPTIDASES; CONTROLLED GENE-EXPRESSION; SEC-INDEPENDENT PROTEIN; METABOLIC FLUX ANALYSIS; LARGE-SCALE PRODUCTION; HIGH-LEVEL SECRETION; BACILLUS-SUBTILIS; STREPTOMYCES-LIVIDANS; RECOMBINANT PROTEIN;
D O I
10.1007/82_2016_49
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A number of Gram-positive bacteria are important players in industry as producers of a diverse array of economically interesting metabolites and proteins. As discussed in this overview, several Gram-positive bacteria are valuable hosts for the production of heterologous proteins. In contrast to Gram-negative bacteria, proteins secreted by Gram-positive bacteria are released into the culture medium where conditions for correct folding are more appropriate, thus facilitating the isolation and purification of active proteins. Although seven different protein secretion pathways have been identified in Gram-positive bacteria, the majority of heterologous proteins are produced via the general secretion or Sec pathway. Not all proteins are equally well secreted, because heterologous protein production often faces bottlenecks including hampered secretion, susceptibility to proteases, secretion stress, and metabolic burden. These bottlenecks are associated with reduced yields leading to non-marketable products. In this chapter, besides a general overview of the different protein secretion pathways, possible hurdles that may hinder efficient protein secretion are described and attempts to improve yield are discussed including modification of components of the Sec pathway. Attention is also paid to omics-based approaches that may offer a more rational approach to optimize production of heterologous proteins.
引用
收藏
页码:267 / 308
页数:42
相关论文
共 199 条
[1]   PPE and PE_PGRS proteins of Mycobacterium marinum are transported via the type VII secretion system ESX-5 [J].
Abdallah, Abdallah M. ;
Verboom, Theo ;
Weerdenburg, Eveline M. ;
Gey van Pittius, Nicolaas C. ;
Mahasha, Phetole W. ;
Jimenez, Connie ;
Parra, Marcela ;
Cadieux, Nathalie ;
Brennan, Michael J. ;
Appelmelk, Ben J. ;
Bitter, Wilbert .
MOLECULAR MICROBIOLOGY, 2009, 73 (03) :329-340
[2]   Immunoregulatory functions and expression patterns of PE/PPE family members: Roles in pathogenicity and impact on anti-tuberculosis vaccine and drug design [J].
Ahmed, Asma ;
Das, Arghya ;
Mukhopadhyay, Sangita .
IUBMB LIFE, 2015, 67 (06) :414-427
[3]   Identification of the Streptococcus mutans LytST two-component regulon reveals its contribution to oxidative stress tolerance [J].
Ahn, Sang-Joon ;
Qu, Ming-Da ;
Roberts, Elisha ;
Burne, Robert A. ;
Rice, Kelly C. .
BMC MICROBIOLOGY, 2012, 12
[4]   High-level secretion of a recombinant protein to the culture medium with a Bacillus subtilis twin-arginine translocation system in Escherichia coli [J].
Albiniak, Anna M. ;
Matos, Cristina F. R. O. ;
Branston, Steven D. ;
Freedman, Robert B. ;
Keshavarz-Moore, Eli ;
Robinson, Colin .
FEBS JOURNAL, 2013, 280 (16) :3810-3821
[5]   In vitro degradation of oxalate by recombinant Lactobacillus plantarum expressing heterologous oxalate decarboxylase [J].
Anbazhagan, K. ;
Sasikumar, P. ;
Gomathi, S. ;
Priya, H. P. ;
Selvam, G. S. .
JOURNAL OF APPLIED MICROBIOLOGY, 2013, 115 (03) :880-887
[6]   Protein secretion biotechnology in Gram-positive bacteria with special emphasis on Streptomyces lividans [J].
Anne, Jozef ;
Vrancken, Kristof ;
Van Mellaert, Lieve ;
Van Impe, Jan ;
Bernaerts, Kristel .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2014, 1843 (08) :1750-1761
[7]   Recombinant protein production and streptomycetes [J].
Anne, Jozef ;
Maldonado, Barbara ;
Van Impe, Jan ;
Van Mellaert, Lieve ;
Bernaerts, Kristel .
JOURNAL OF BIOTECHNOLOGY, 2012, 158 (04) :159-167
[8]  
Ayala J. C., 2015, Asian Pacific Journal of Tropical Disease, V5, P772
[9]   Quantitative export of a reporter protein, GFP, by the twin-arginine translocation pathway in Escherichia coli [J].
Barrett, CML ;
Ray, N ;
Thomas, JD ;
Robinson, C ;
Bolhuis, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 304 (02) :279-284
[10]   Compensatory role of PspA, a member of the phage shock protein operon, in rpoE mutant Salmonella enterica serovar Typhimurium [J].
Becker, LA ;
Bang, IS ;
Crouch, ML ;
Fang, FC .
MOLECULAR MICROBIOLOGY, 2005, 56 (04) :1004-1016