Regional distribution and the dynamics of n-decanoyl ghrelin, another acyl-form of ghrelin, upon fasting in rodents

被引：18

作者：

Hiejima, Hiroshi ^{[1
,2
]}

Nishi, Yoshihiro ^{[1
]}

Hosoda, Hiroshi ^{[6
]}

Yoh, Junko ^{[1
]}

Mifune, Hiroharu ^{[4
]}

Satou, Motoyasu ^{[7
]}

Sugimoto, Hiroyuki ^{[7
]}

Chiba, Seiichi ^{[8
]}

Kawahara, Yukie ^{[3
]}

Tanaka, Eiichiro ^{[1
]}

Yoshimatsu, Hironobu ^{[8
]}

Uchimura, Naohisa ^{[2
]}

Kangawa, Kenji ^{[6
]}

Kojima, Masayasu ^{[5
]}

机构：

[1] Kurume Univ, Sch Med, Dept Physiol, Fukuoka 8300011, Japan

[2] Kurume Univ, Sch Med, Dept Psychiat, Fukuoka 8300011, Japan

[3] Kurume Univ, Sch Med, Dept Pharmacol, Fukuoka 8300011, Japan

[4] Kurume Univ, Sch Med, Inst Anim Experimentat, Fukuoka 8300011, Japan

[5] Kurume Univ, Inst Life Sci, Div Mol Genet, Fukuoka 8390807, Japan

[6] Natl Cardiovasc Ctr, Res Inst, Dept Biochem, Osaka, Japan

[7] Dokkyo Med Univ, Sch Med, Dept Biochem, Mibu, Tochigi, Japan

[8] Oita Univ, Sch Med, Dept Internal Med, Oita 87011, Japan

来源：

REGULATORY PEPTIDES | 2009年 / 156卷 / 1-3期

关键词：

Acylation; Fasting; Fatty acids; Food consumption; Gastrointestinal hormone; Ghrelin; Immunofluorescence; Radioimmunoassay; RP-HPLC; GROWTH-HORMONE SECRETAGOGUES; FATTY-ACIDS; RECEPTOR; PEPTIDE; STOMACH; IDENTIFICATION; PURIFICATION; LEPTIN; MICE;

D O I：

10.1016/j.regpep.2009.05.003

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

n-Decanoyl ghrelin (D-ghrelin), a member of ghrelin-derived peptides, is found in plasma and the stomach; however, there have so far been no studies describing its dynamics. A D-ghrelin-specific radioimmunoassay was established to examine the tissue distribution and the kinetics of D-ghrelin in mice. The effect of D-ghrelin on food intake was also examined and compared to n-octanoyl ghrelin (O-ghrelin). D-ghrelin was detected throughout the gastrointestinal tissue and plasma with highest level in the stomach. An immunofluorescent study revealed the co-localization of D- and C-ghrelin in the same stomach cells. Upon fasting, the levels of D-ghrelin in the stomach and plasma significantly increased, while that of O-ghrelin in the stomach declined. D-ghrelin increased the 2 h food consumption in mice as O-ghrelin does. These findings indicate that D-ghrelin is mainly produced in the stomach to work in concert with O-ghrelin. The different kinetics of D- and O-ghrelin in the stomach upon fasting implies the possibility of D-ghrelin-specific bioregulation. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：47 / 56

页数：10

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