In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy

被引：2

作者：

Wang, Zhibin ^{[1
]}

Wei, Lin ^{[1
]}

He, Yi ^{[1
]}

Li, Xiqi ^{[1
]}

Shi, Guohua ^{[1
]}

Zhang, Yudong ^{[1
]}

机构：

[1] Chinese Acad Sci, Key Lab Adapt Opt, Chengdu 610209, Peoples R China

来源：

TWELFTH INTERNATIONAL CONFERENCE ON PHOTONICS AND IMAGING IN BIOLOGY AND MEDICINE (PIBM 2014) | 2014年 / 9230卷

关键词：

Adaptive optics; confocal microscopy; in vivo imaging; vascular imaging; aberration correct;

D O I：

10.1117/12.2068823

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures.

引用

页数：5

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