Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography

被引：116

作者：

Li, SP ^{[1
]}

Li, P

Lai, CM

Gong, YX

Kan, KKW

Dong, TTX

Tsim, KWK

Wang, YT

机构：

[1] Univ Macau, Inst Chinese Med Sci, Taipa, Macau, Peoples R China

[2] Nanjing Univ Tradit Chinese Med, Natl Stand Lab Tradit Chinese Med, Nanjing, Peoples R China

[3] Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China

[4] Hong Kong Univ Sci & Technol, Biotechnol Res Inst, Hong Kong, Hong Kong, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1036卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Cordyceps spp; pharmaceutical analysis; ergosterol; sterols; nucleosides;

D O I：

10.1016/j.chroma.2004.02.080

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method is described for the simultaneous determination of ergosterol, nucleosides and their bases in Cordyceps. The samples were extracted by using pressurised liquid extraction (PLE). The effects of experimental variables, such as solvent, temperature, static extraction time and cycles, on PLE efficiency have been studied. The results showed a strong influence of the solvent and temperature on extraction efficiency of PLE. The determination was achieved by high-performance liquid chromatography (HPLC) using a Zorbax NH2 analytical column (250 x 4.6 mm i.d., 5 mum) with diode-array detector (DAD). The automated preparation of the sample permits a very fast analysis which is an important goal for routine purpose. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：239 / 243

页数：5

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