Reactive Oxygen Species and Boar Sperm Function

被引:175
作者
Awda, Basim J. [1 ]
Mackenzie-Bell, Meghan [1 ]
Buhr, Mary M. [1 ]
机构
[1] Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
acrosome reaction; cryopreservation; gamete biology; hydrogen peroxide; lipid peroxidation; phospholipase A; signal transduction; sperm capacitation; sperm motility and transport; tyrosine phosphorylation; PROTEIN-TYROSINE PHOSPHORYLATION; SIGNAL-TRANSDUCTION PATHWAYS; LIPID-PEROXIDATION; SUPEROXIDE-DISMUTASE; HUMAN SPERMATOZOA; HYDROGEN-PEROXIDE; REDOX REGULATION; KINASE-ACTIVITY; COLD SHOCK; CAPACITATION;
D O I
10.1095/biolreprod.109.076471
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Boar spermatozoa are very susceptible to reactive oxygen species (ROS), but ROS involvement in damage and/or capacitation is unclear. The impact of exposing fresh boar spermatozoa to an ROS-generating system (xanthine/xanthine oxidase; XA/XO) on sperm ROS content, membrane lipid peroxidation, phospholipase (PL) A activity, and motility, viability, and capacitation was contrasted to ROS content and sperm function after cryopreservation. Exposing boar sperm (n = 4-5 ejaculates) to the ROS-generating system for 30 min rapidly increased hydrogen peroxide (H2O2) and lipid peroxidation in all sperm, increased PLA in dead sperm, and did not affect intracellular O-2(center dot-) (flow cytometry of sperm labeled with 2,7'-dichlorodihydrofluorscein diacetate, BODIPY 581/591 C11, bis-BODIPY-FL C11, hydroethidine, respectively; counterstained for viability). Sperm viability remained high, but sperm became immotile. Cryopreservation decreased sperm motility, viability, and intracellular O-2(center dot-) significantly, but did not affect H2O2. As expected, more sperm incubated in capacitating media than Beltsville thawing solution buffer underwent acrosome reactions and protein tyrosine phosphorylation (four proteins, 58-174 kDa); which proteins were tyrosine phosphorylated was pH dependent. Pre-exposing sperm to the ROS-generating system increased the percentage of sperm that underwent acrosome reactions after incubation in capacitating conditions (P < 0.025), and decreased capacitation-dependent increases in two tyrosine-phosphorylated proteins (P <= 0.035). In summary, H2O2 is the major free radical mediating direct ROS effects, but not cryopreservation changes, on boar sperm. Boar sperm motility, acrosome integrity, and lipid peroxidation are more sensitive indicators of oxidative stress than viability and PLA activity. ROS may stimulate the acrosome reaction in boar sperm through membrane lipid peroxidation and PLA activation.
引用
收藏
页码:553 / 561
页数:9
相关论文
共 92 条
[1]  
AITKEN RJ, 1995, J CELL SCI, V108, P2017
[2]   A FREE-RADICAL THEORY OF MALE-INFERTILITY [J].
AITKEN, RJ .
REPRODUCTION FERTILITY AND DEVELOPMENT, 1994, 6 (01) :19-24
[3]  
AITKEN RJ, 1993, J REPROD FERTIL, V97, P441
[4]   Free radicals, lipid peroxidation and sperm function [J].
Aitken, RJ .
REPRODUCTION FERTILITY AND DEVELOPMENT, 1995, 7 (04) :659-668
[5]   GENERATION OF REACTIVE OXYGEN SPECIES, LIPID-PEROXIDATION, AND HUMAN-SPERM FUNCTION [J].
AITKEN, RJ ;
CLARKSON, JS ;
FISHEL, S .
BIOLOGY OF REPRODUCTION, 1989, 41 (01) :183-197
[6]  
AITKEN RJ, 1987, J REPROD FERTIL, V81, P459
[7]   TAURINE, HYPOTAURINE, EPINEPHRINE AND ALBUMIN INHIBIT LIPID-PEROXIDATION IN RABBIT SPERMATOZOA AND PROTECT AGAINST LOSS OF MOTILITY [J].
ALVAREZ, JG ;
STOREY, BT .
BIOLOGY OF REPRODUCTION, 1983, 29 (03) :548-555
[8]  
ALVAREZ JG, 1992, J ANDROL, V13, P232
[10]   Signal transduction pathways in human spermatozoa [J].
Baldi, E ;
Luconi, M ;
Bonaccorsi, L ;
Forti, G .
JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 2002, 53 (1-2) :121-131