Descriptive analysis of bacterial profile, physicochemical and sensory characteristics of grape juice containing Saccharomyces cerevisiae cell wall-coated probiotic microcapsules during storage

In this study the feasibility of incorporation of probiotic microcapsules coated with fragmented yeast cell wall in grape juice was evaluated during 60days at 4 degrees C. Lactobacillus acidophilus and Bifidobacterium bifidum were encapsulated in alginate microbeads and coated with fragmented Saccharomyces cerevisiae cell wall and calcium alginate and were added into grape juice. At the end of storage, the survival of probiotics was higher than recommended minimum value (10(7)cfu mL(-1)) and the results demonstrated that applying yeast cell wall layer for L.acidophilus microcapsules significantly enhanced its survival while did not affect the survival of B.bifidum (P>0.05). Generally, probiotic grape juice showed decrease in degrees Brix, pH and colour and increase in acidity and turbidity during storage and the presence of yeast wall layer had no significant effect on its properties expect colour and turbidity. Overall acceptance of grape juices containing yeast cell wall-coated microcapsules scored the least.