No evidence for enhanced parvalbumin concentration in light muscle of transgenic coho salmon (Oncorhynchus kisutch)

被引：0
作者：
Rehbein, Hartmut Walter [1 ]
Devlin, Robert H. [2 ]
机构：
[1] Max Rubner Inst, Fed Res Inst Nutr & Food, Dept Safety & Qual Milk & Fish Prod, D-22767 Hamburg, Germany
[2] Fisheries & Oceans Canada, W Vancouver, BC V7V 1N6, Canada
来源：
EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2009年 / 229卷 / 04期
关键词：
Transgenic fish; Coho salmon; Parvalbumin; DYE STAINS-ALL; GROWTH; FISH; IDENTIFICATION; PROTEINS; TILAPIA; TROUT;
D O I：
10.1007/s00217-009-1086-0
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The expression of parvalbumin, a major allergenic protein of fish muscle, was determined in rapidly growing transgenic coho salmon (Oncorhynchus mykiss) containing the growth hormone (GH) gene construct OnMTGH1. Three different methods for parvalbumin analysis were used: (1) measurement of the mRNA concentration by real-time RT-PCR; (2) isoelectric focusing of sarcoplasmic proteins; (3) assessment of amount of heat-stable, Ca(2+)-binding sarcoplasmic proteins by measuring absorbance spectra of "stains-all"aEuro"protein complexes. Compared to non-transgenic coho salmon, no indication for enhanced expression of parvalbumin in transgenic fish was found either at the mRNA or at the protein level.
引用
收藏
页码：579 / 584
页数：6
相关论文
共 50 条
  • [41] Fast-growing growth hormone transgenic coho salmon (Oncorhynchus kisutch) show a lower incidence of vaterite deposition and malformations in sagittal otoliths
    Chalan, Irvin
    Solsona, Laia
    Coll-Llado, Clara
    Webb, Paul B.
    Sakhrani, Dionne
    Devlin, Robert H.
    de la Serrana, Daniel Garcia
    JOURNAL OF EXPERIMENTAL BIOLOGY, 2022, 225 (19)
  • [42] Evaluating cardiac oxygen limitation as a mechanism for female-biased mortality in coho salmon (Oncorhynchus kisutch)
    Little, A. G.
    Prystay, T. S.
    Hardison, E. A.
    Dressler, T.
    Kraskura, K.
    Cooke, S. J.
    Patterson, D. A.
    Hinch, S. G.
    Eliason, E. J.
    CANADIAN JOURNAL OF ZOOLOGY, 2023, : 163 - 171
  • [43] Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
    Marquez, Lorenzo
    Hernandez, Adrian J.
    Carvalho, Edison S. M.
    Morales, Gabriel A.
    Dantagnan, Patricio
    Diaz, Manuel
    Moyano, Francisco J.
    LATIN AMERICAN JOURNAL OF AQUATIC RESEARCH, 2019, 47 (05): : 860 - 864
  • [44] Identification, development, and characterization of three molecular markers associated to spawning date in Coho salmon (Oncorhynchus kisutch)
    Araneda, Cristian
    Lam, Natalia
    Diaz, Nelson F.
    Cortez, Soledad
    Perez, Claudio
    Neira, Roberto
    Iturra, Patricia
    AQUACULTURE, 2009, 296 (1-2) : 21 - 26
  • [45] Methemoglobin determination by multi-component analysis in coho salmon (Oncorhynchus kisutch) possessing unstable hemoglobin
    Blair, Stephanie
    Barlow, Clyde
    Martin, Erin
    Schumaker, Ruth
    McIntyre, Jenifer
    METHODSX, 2020, 7
  • [46] Ontogenetic changes in photoreceptor opsin gene expression in coho salmon (Oncorhynchus kisutch, Walbaum)
    Temple, S. E.
    Veldhoen, K. M.
    Phelan, J. T.
    Veldhoen, N. J.
    Hawryshyn, C. W.
    JOURNAL OF EXPERIMENTAL BIOLOGY, 2008, 211 (24) : 3879 - 3888
  • [47] Effects of exogenous thyroid hormones on visual pigment composition in coho salmon (Oncorhynchus kisutch)
    Temple, Shelby E.
    Ramsden, Samuel D.
    Haimberger, Theodore J.
    Veldhoen, Kathy M.
    Veldhoen, Nik J.
    Carter, Nicolette L.
    Roth, Wolff-Michael
    Hawryshyn, Craig W.
    JOURNAL OF EXPERIMENTAL BIOLOGY, 2008, 211 (13) : 2134 - 2143
  • [48] Seasonal dynamics of juvenile coho salmon (Oncorhynchus kisutch) in wetlands of the North Thompson River, British Columbia
    Milner, Brittany L.
    Braun, Douglas
    Moore, Jonathan W.
    Martens, Amanda M.
    Loscerbo, Daniella
    Naman, Sean
    CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 2025, 82
  • [49] Domestication and growth hormone transgenesis cause similar changes in gene expression in coho salmon (Oncorhynchus kisutch)
    Devlin, Robert H.
    Sakhrani, Dionne
    Tymchuk, Wendy E.
    Rise, Matthew L.
    Goh, Benjamin
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (09) : 3047 - 3052
  • [50] Effects of dietary exposure of 4-nonylphenol on growth and smoltification of juvenile coho salmon (Oncorhynchus kisutch)
    Keen, PL
    Higgs, DA
    Hall, KJ
    Ikonomou, M
    SCIENCE OF THE TOTAL ENVIRONMENT, 2005, 349 (1-3) : 81 - 94
12345