No evidence for enhanced parvalbumin concentration in light muscle of transgenic coho salmon (Oncorhynchus kisutch)

被引：0
|
作者：
Rehbein, Hartmut Walter [1 ]
Devlin, Robert H. [2 ]
机构：
[1] Max Rubner Inst, Fed Res Inst Nutr & Food, Dept Safety & Qual Milk & Fish Prod, D-22767 Hamburg, Germany
[2] Fisheries & Oceans Canada, W Vancouver, BC V7V 1N6, Canada
来源：
EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2009年 / 229卷 / 04期
关键词：
Transgenic fish; Coho salmon; Parvalbumin; DYE STAINS-ALL; GROWTH; FISH; IDENTIFICATION; PROTEINS; TILAPIA; TROUT;
D O I：
10.1007/s00217-009-1086-0
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The expression of parvalbumin, a major allergenic protein of fish muscle, was determined in rapidly growing transgenic coho salmon (Oncorhynchus mykiss) containing the growth hormone (GH) gene construct OnMTGH1. Three different methods for parvalbumin analysis were used: (1) measurement of the mRNA concentration by real-time RT-PCR; (2) isoelectric focusing of sarcoplasmic proteins; (3) assessment of amount of heat-stable, Ca(2+)-binding sarcoplasmic proteins by measuring absorbance spectra of "stains-all"aEuro"protein complexes. Compared to non-transgenic coho salmon, no indication for enhanced expression of parvalbumin in transgenic fish was found either at the mRNA or at the protein level.
引用
收藏
页码：579 / 584
页数：6
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