Field performance of terpene-producing Camelina sativa

被引:12
作者
Augustin, Joerg M. [1 ,2 ]
Brock, Jordan R. [1 ,3 ]
Augustin, Megan M. [1 ]
Wellinghoff, Rachel L. [1 ,4 ]
Shipp, Matthew [1 ,5 ]
Higashi, Yasuhiro [1 ,6 ]
Kumssa, Tadele T. [7 ,8 ]
Cahoong, Edgar B. [7 ]
Kutchan, Toni M. [1 ]
机构
[1] Donald Danforth Plant Sci Ctr, 975 North Warson Rd, St Louis, MO 63132 USA
[2] Elemental Enzymes, 1685 Galt Ind Blvd, St Louis, MO 63132 USA
[3] Washington Univ, Dept Biol, 1 Brookings Dr, St Louis, MO 63130 USA
[4] Cofactor Genom, 4044 Clayton Ave, St Louis, MO 63110 USA
[5] KWS, 1005 N Warson Rd, St Louis, MO 63132 USA
[6] RIKEN, Ctr Sustainable Resource Sci, Tsurumi Ku, 1-7-22 Suehiro, Yokohama, Kanagawa 2300045, Japan
[7] Univ Nebraska, 1400 R St, Lincoln, NE 68588 USA
[8] Noble Res Inst, 2510 Sam Noble Pkwy, Ardmore, OK 73401 USA
关键词
Terpene; (+)-delta-cadinene; (4S)-limonene; (+)-5-epi-aristolochene; Field trial; Camelina sativa; FISH-OIL; OILSEED CROPS; L; CRANTZ; TRANSCRIPTOME; PLANT; HYBRIDIZATION; PLATFORM;
D O I
10.1016/j.indcrop.2019.04.061
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Bioengineered lines of the low-input oil seed crop Camelina sativa (Camelina), augmented to accumulate the monoterpene (4S)-limonene, the sesquiterpene ( + )-delta-cadinene, or the sesquiterpene ( + )-5-epi-aristolochene in seed, were evaluated for two growing seasons under field conditions to determine performance of the introduced traits in an agricultural setup including the effects on overall plant fitness, and total seed yield. Field-grown Camelina plants were further compared to greenhouse-grown plants to evaluate commonalities and differences resulting from cultivation under either controlled or agriculturally relevant growth conditions. Morphological appearance and plant height differed marginally between transgenic and wild-type plants under both greenhouse and field conditions, indicating low impact of the terpene production traits toward overall plant fitness. Total seed yield, however, was independent of the growth conditions and was reduced in Camelina lines producing (4S)-limonene by 48% on average and by 30% on average for ( + )-5-epi-aristolochene producing lines. Conversely, ( + )-delta-cadinene producing Camelina seed yields remained wild-type equivalent. Additional investigations included seed terpene accumulation, seed oil amount, and seed fatty acid composition. Terpene accumulation was reduced up to 22% for field-grown plants as compared to greenhouse-grown plants. Seed oil amounts were similar under greenhouse and field conditions but were consistently lowered by 2-5% for terpene producing lines. Similarly, seed oil composition remained stable under both field and greenhouse conditions, but generally favored a more energy dense phenotype in terpene producing Camelina lines. Lastly, outcrossing of transgenic traits to adjacent wild-type Camelina plants was observed under field but not greenhouse conditions.
引用
收藏
页码:50 / 58
页数:9
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