An attenuated live vaccine based on highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) protects piglets against HP-PRRS

被引：127

作者：

Tian, Zhi-Jun ^{[2
]}

An, Tong-Qing ^{[2
]}

Zhou, Yan-Jun ^{[2
]}

Peng, Jin-Mei ^{[2
]}

Hu, Shou-Ping ^{[2
]}

Wei, Tian-Chao ^{[2
]}

Jiang, Yi-Feng ^{[2
]}

Xiao, Yan ^{[2
]}

Tong, Guang-Zhi ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China

[2] Chinese Acad Agr Sci, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Div Swine Infect Dis, Harbin 150001, Peoples R China

来源：

VETERINARY MICROBIOLOGY | 2009年 / 138卷 / 1-2期

关键词：

Highly pathogenic porcine reproductive and respiratory syndrome; Vaccine; Immunization; GP5;

D O I：

10.1016/j.vetmic.2009.03.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Porcine infections with highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) cause significant morbidity and mortality and currently there are no effective vaccines for disease prevention. An attenuated strain, HuN4-F112, was obtained by passaging the HP-PRRSV HuN4 on Marc-145 cells (112th-passage). PRRSV-free pigs were inoculated intramuscularly with HuN4-F112 (10(2.0),10(3.0),10(4.0),10(5.0) and 10(6.0) TCID50 for groups 1-5, respectively). The groups 3-5 could resist the lethal challenge and did not show any obvious changes in body temperature nor clinical signs throughout the experiment, the pathological lesions were milder and the gained weight at a greater rate (P < 0.05), compared to group 1 and control. Sequence analysis of the HuN4 passages showed a conserved epitope in GP5 protein was mutated ((196)QWGRL/P-200 -> (196)RWGRL/P-200), as a result the monoclonal antibody could not recognize the HuN4-F112 any more. These results suggested that the HuN4-F112 could protect piglets from lethal challenge and might be a candidate vaccine against the HP-PRRSV. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：34 / 40

页数：7

共 16 条

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