A stress-induced response complex (SIRC) shuttles miRNAs, siRNAs, and oligonucleotides to the nucleus

被引：31
作者：
Castanotto, Daniela [1 ]
Zhang, Xiaowei [1 ]
Alluin, Jessica [2 ]
Zhang, Xizhe [3 ]
Rueger, Jacqueline [1 ]
Armstrong, Brian [4 ]
Rossi, John [2 ]
Riggs, Arthur [3 ]
Stein, C. A. [1 ,2 ]
机构：
[1] City Hope Natl Med Ctr, Dept Med Oncol, Duarte, CA 91010 USA
[2] City Hope Natl Med Ctr, Dept Mol & Cellular Biol, Duarte, CA 91010 USA
[3] City Hope Natl Med Ctr, Dept Diabet & Metab Dis, Duarte, CA 91010 USA
[4] City Hope Natl Med Ctr, Dept Neurosci, Duarte, CA 91010 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2018年 / 115卷 / 25期
关键词：
miRNA; SSO; stress granules; FUS; YB1; BOX-BINDING PROTEIN-1; RNA INTERFERENCE; MESSENGER-RNA; PHOSPHOROTHIOATE OLIGONUCLEOTIDES; ANTISENSE OLIGONUCLEOTIDES; OXIDATIVE STRESS; MAMMALIAN-CELLS; GRANULES; YB-1; BODIES;
D O I：
10.1073/pnas.1721346115
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Although some information is available for specific subsets of miRNAs and several factors have been shown to bind oligonucleotides (ONs), no general transport mechanism for these molecules has been identified to date. In this work, we demonstrate that the nuclear transport of ONs, siRNAs, and miRNAs responds to cellular stress. Furthermore, we have identified a stress-induced response complex (SIRC), which includes Ago-1 and Ago-2 in addition to the transcription and splicing regulators YB1, CTCF, FUS, Smad1, Smad3, and Smad4. The SIRC transports endogenous miRNAs, siRNAs, and ONs to the nucleus. We show that cellular stress can significantly increase ON-or siRNA-directed splicing switch events and endogenous miRNA targeting of nuclear RNAs.
引用
收藏
页码：E5756 / E5765
页数：10
相关论文
共 57 条
[11]   A novel class of oligonucleotide analogues containing 2′-O,3′-C-linked [3.2.0]bicycloarabinonucleoside monomers:: Synthesis, thermal affinity studies, and molecular modeling [J].
Christensen, NK ;
Petersen, M ;
Nielsen, P ;
Jacobsen, JP ;
Olsen, CE ;
Wengel, J .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (22) :5458-5463
[12]   Cell stress is related to re-localization of Argonaute 2 and to decreased RNA interference in human cells [J].
Detzer, Anke ;
Engel, Christina ;
Wuensche, Winfried ;
Sczakiel, Georg .
NUCLEIC ACIDS RESEARCH, 2011, 39 (07) :2727-2741
[13]   ALS-associated fused in sarcoma (FUS) mutations disrupt Transportin-mediated nuclear import [J].
Dormann, Dorothee ;
Rodde, Ramona ;
Edbauer, Dieter ;
Bentmann, Eva ;
Fischer, Ingeborg ;
Hruscha, Alexander ;
Than, Manuel E. ;
Mackenzie, Ian R. A. ;
Capell, Anja ;
Schmid, Bettina ;
Neumann, Manuela ;
Haass, Christian .
EMBO JOURNAL, 2010, 29 (16) :2841-2857
[14]   Y-box-binding protein 1 (YB-1) and its functions [J].
Eliseeva, I. A. ;
Kim, E. R. ;
Guryanov, S. G. ;
Ovchinnikov, L. P. ;
Lyabin, D. N. .
BIOCHEMISTRY-MOSCOW, 2011, 76 (13) :1402-1433
[15]   miRNAs at the interface of cellular stress and disease [J].
Emde, Anna ;
Hornstein, Eran .
EMBO JOURNAL, 2014, 33 (13) :1428-1437
[16]   Protein detection using proximity-dependent DNA ligation assays [J].
Fredriksson, S ;
Gullberg, M ;
Jarvius, J ;
Olsson, C ;
Pietras, K ;
Gústafsdóttir, SM ;
Östman, A ;
Landegren, U .
NATURE BIOTECHNOLOGY, 2002, 20 (05) :473-477
[17]   RNAi Factors Are Present and Active in Human Cell Nuclei [J].
Gagnon, Keith T. ;
Li, Liande ;
Chu, Yongjun ;
Janowski, Bethany A. ;
Corey, David R. .
CELL REPORTS, 2014, 6 (01) :211-221
[18]   The rules and roles of nucleocytoplasmic shuttling proteins [J].
Gama-Carvalho, M ;
Carmo-Fonseca, M .
FEBS LETTERS, 2001, 498 (2-3) :157-163
[19]   A superfamily of proteins that contain the cold-shock domain [J].
Graumann, PL ;
Marahiel, MA .
TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (08) :286-290
[20]   Natural RNA circles function as efficient microRNA sponges [J].
Hansen, Thomas B. ;
Jensen, Trine I. ;
Clausen, Bettina H. ;
Bramsen, Jesper B. ;
Finsen, Bente ;
Damgaard, Christian K. ;
Kjems, Jorgen .
NATURE, 2013, 495 (7441) :384-388
123456