A stress-induced response complex (SIRC) shuttles miRNAs, siRNAs, and oligonucleotides to the nucleus

被引:31
作者
Castanotto, Daniela [1 ]
Zhang, Xiaowei [1 ]
Alluin, Jessica [2 ]
Zhang, Xizhe [3 ]
Rueger, Jacqueline [1 ]
Armstrong, Brian [4 ]
Rossi, John [2 ]
Riggs, Arthur [3 ]
Stein, C. A. [1 ,2 ]
机构
[1] City Hope Natl Med Ctr, Dept Med Oncol, Duarte, CA 91010 USA
[2] City Hope Natl Med Ctr, Dept Mol & Cellular Biol, Duarte, CA 91010 USA
[3] City Hope Natl Med Ctr, Dept Diabet & Metab Dis, Duarte, CA 91010 USA
[4] City Hope Natl Med Ctr, Dept Neurosci, Duarte, CA 91010 USA
关键词
miRNA; SSO; stress granules; FUS; YB1; BOX-BINDING PROTEIN-1; RNA INTERFERENCE; MESSENGER-RNA; PHOSPHOROTHIOATE OLIGONUCLEOTIDES; ANTISENSE OLIGONUCLEOTIDES; OXIDATIVE STRESS; MAMMALIAN-CELLS; GRANULES; YB-1; BODIES;
D O I
10.1073/pnas.1721346115
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Although some information is available for specific subsets of miRNAs and several factors have been shown to bind oligonucleotides (ONs), no general transport mechanism for these molecules has been identified to date. In this work, we demonstrate that the nuclear transport of ONs, siRNAs, and miRNAs responds to cellular stress. Furthermore, we have identified a stress-induced response complex (SIRC), which includes Ago-1 and Ago-2 in addition to the transcription and splicing regulators YB1, CTCF, FUS, Smad1, Smad3, and Smad4. The SIRC transports endogenous miRNAs, siRNAs, and ONs to the nucleus. We show that cellular stress can significantly increase ON-or siRNA-directed splicing switch events and endogenous miRNA targeting of nuclear RNAs.
引用
收藏
页码:E5756 / E5765
页数:10
相关论文
共 57 条
[1]   Kinesin molecular motor Eg5 functions during polypeptide synthesis [J].
Bartoli, Kristen M. ;
Jakovljevic, Jelena ;
Woolford, John L., Jr. ;
Saunders, William S. .
MOLECULAR BIOLOGY OF THE CELL, 2011, 22 (18) :3420-3430
[2]   Antiproliferative activity of G-rich oligonucleotides correlates with protein binding [J].
Bates, PJ ;
Kahlon, JB ;
Thomas, SD ;
Trent, JO ;
Miller, DM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (37) :26369-26377
[3]   Transforming Growth Factor β Promotes Complexes between Smad Proteins and the CCCTC-binding Factor on the H19 Imprinting Control Region Chromatin [J].
Bergstrom, Rosita ;
Savary, Katia ;
Moren, Anita ;
Guibert, Sylvain ;
Heldin, Carl-Henrik ;
Ohlsson, Rolf ;
Moustakas, Aristidis .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (26) :19727-19737
[4]   Links between the Oncoprotein YB-1 and Small Non-Coding RNAs in Breast Cancer [J].
Blenkiron, Cherie ;
Hurley, Daniel G. ;
Fitzgerald, Sandra ;
Print, Cristin G. ;
Lasham, Annette .
PLOS ONE, 2013, 8 (11)
[5]   Eukaryotic Stress Granules: The Ins and Outs of Translation [J].
Buchan, J. Ross ;
Parker, Roy .
MOLECULAR CELL, 2009, 36 (06) :932-941
[6]   A cytoplasmic pathway for gapmer antisense oligonucleotide-mediated gene silencing in mammalian cells [J].
Castanotto, Daniela ;
Lin, Min ;
Kowolik, Claudia ;
Wang, LiAnn ;
Ren, Xiao-Qin ;
Soifer, Harris S. ;
Koch, Troels ;
Hansen, Bo Rode ;
Oerum, Henrik ;
Armstrong, Brian ;
Wang, Zhigang ;
Bauer, Paul ;
Rossi, John ;
Stein, C. A. .
NUCLEIC ACIDS RESEARCH, 2015, 43 (19) :9350-9361
[7]   CRM1 mediates nuclear-cytoplasmic shuttling of mature microRNAs [J].
Castanotto, Daniela ;
Lingeman, Robert ;
Riggs, Arthur D. ;
Rossi, John J. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (51) :21655-21659
[8]   MicroRNA in Control of Gene Expression: An Overview of Nuclear Functions [J].
Catalanotto, Caterina ;
Cogoni, Carlo ;
Zardo, Giuseppe .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016, 17 (10)
[9]   From an old remedy to a magic bullet: molecular mechanisms underlying the therapeutic effects of arsenic in fighting leukemia [J].
Chen, Sai-Juan ;
Zhou, Guang-Biao ;
Zhang, Xiao-Wei ;
Mao, Jian-Hua ;
de The, Hugues ;
Chen, Zhu .
BLOOD, 2011, 117 (24) :6425-6437
[10]   Physical and functional interaction between two pluripotent proteins, the Y-box DNA/RNA-binding factor, YB-1, and the multivalent zinc finger factor, CTCF [J].
Chernukhin, IV ;
Shamsuddin, S ;
Robinson, AF ;
Carne, AF ;
Paul, A ;
El-Kady, AI ;
Lobanenkov, VV ;
Klenova, EM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (38) :29915-29921