mRNA expression and protein-protein interaction (PPI) network analysis of adrenal steroidogenesis in response to exposure to phthalates in rats

被引：13

作者：

Ahmad, Shahzad ^{[1
]}

Sharma, Shikha ^{[1
,3
]}

Afjal, Mohd Amir ^{[1
]}

Habib, Haroon ^{[1
]}

Akhter, Juheb ^{[1
]}

Goswami, Poonam ^{[1
]}

Parvez, Suhel ^{[1
]}

Akhtar, Mohammad ^{[2
]}

Raisuddin, Sheikh ^{[1
]}

机构：

[1] Jamia Hamdard, Dept Med Elementol & Toxicol, New Delhi 110062, India

[2] Jamia Hamdard, Dept Pharmacol, New Delhi 110062, India

[3] Indiana Univ, Sch Publ Hlth, Dept Environm & Occupat Hlth, Bloomington, IN 47408 USA

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2022年 / 89卷

关键词：

Environmental endocrine disruptors; Glucocorticoid biosynthesis; Phthalate esters; Protein-protein interaction; Inflammatory cytokines; Gene ontology; N-BUTYL PHTHALATE; IN-UTERO EXPOSURE; REPRODUCTIVE TOXICITY; GENE-EXPRESSION; CHEMICALS; DEHP; TESTOSTERONE; DISRUPTION; MECHANISMS; PITUITARY;

D O I：

10.1016/j.etap.2021.103780

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Phthalate esters such as di-butyl phthalate (DBP) and di-ethyl hexyl phthalate (DEHP) used in personal care and consumer products and medical devices have potential to affect human health. We studied the effect of DBP and DEHP on critical enzymes of glucocorticoid biosynthesis pathway in the adrenal gland and pro-inflammatory cytokines in the serum in male Wistar rats. DEHP and DBP treatment altered the mRNA expression of enzymes of glucocorticoid biosynthesis pathway accompanied by a reduction in glucocorticoid production and elevation in the level of glucocorticoid regulated pro-inflammatory cytokines indicating a cascading effect of phthalates. The analysis of PPI (protein - protein interaction) network involving Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) of enzymes through STRING database revealed that all the proteins have the maximum level of interaction with the selected number of proteins. The STRING database analysis together with in vivo data indicates the potential effects of phthalates on various targets of steroidogenesis pathway with a global biological impact.

引用

页数：15

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