Infectivity and complete nucleotide sequence of cucumber fruit mottle mosaic virus isolate Cm cDNA

被引：6
作者：
Rhee, Sun-Ju [1 ]
Hong, Jin-Sung [2 ]
Lee, Gung Pyo [1 ]
机构：
[1] Chung Ang Univ, Dept Integrat Plant Sci, Ansung 456756, South Korea
[2] Kangwon Natl Univ, Dept Appl Biol, Chunchon 200701, South Korea
来源：
ARCHIVES OF VIROLOGY | 2014年 / 159卷 / 07期
基金：
新加坡国家研究基金会;
关键词：
Cucumis melo L; Cucumis sativus L; Full-length cDNA; In vitro transcription; Agro-inoculation; MOLECULAR CHARACTERIZATION; TOBAMOVIRUS; GENE; TOMATO; GENERATION;
D O I：
10.1007/s00705-014-1990-x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Three isolates of cucumber fruit mottle mosaic virus (CFMMV) were collected from melon, cucumber, and pumpkin plants in Korea. A full-length cDNA clone of CFMMV-Cm (melon isolate) was produced and evaluated for infectivity after T7 transcription in vitro (pT7CF-Cm-flc). The complete CFMMV genome sequence of the infectious clone pT7CF-Cm-flc was determined. The genome of CFMMV-Cm consisted of 6,571 nucleotides and shared high nucleotide sequence identity (98.8 %) with the Israel isolate of CFMMV. Based on the infectious clone pT7CF-Cm-flc, a CaMV 35S-promoter driven cDNA clone (p35SCF-Cm-flc) was subsequently constructed and sequenced. Mechanical inoculation with RNA transcripts of pT7CF-Cm-flc and agro-inoculation with p35SCF-Cm-flc resulted in systemic infection of cucumber and melon, producing symptoms similar to those produced by CFMMV-Cm. Progeny virus in infected plants was detected by RT-PCR, western blot assay, and transmission electron microscopy.
引用
收藏
页码：1807 / 1811
页数：5
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