A highly stable, sensitive, regenerable and rapid immunoassay for detecting aflatoxin B1 in corn incorporating covalent AFB1 immobilization and a recombinant Fab antibody

被引：17

作者：

Edupuganti, Soujanya Ratna ^{[1
]}

Edupuganti, Om Prakash ^{[1
]}

Hearty, Stephen ^{[1
]}

O'Kennedy, Richard ^{[1
]}

机构：

[1] Dublin City Univ, Appl Biochem Grp ABG, Sch Biotechnol, Dublin 9, Ireland

来源：

TALANTA | 2013年 / 115卷

关键词：

Covalent immobilization; Passive adsorption; Amide bond; Enzyme-linked immunosorbent assay (ELISA); Re-usability; LINKED-IMMUNOSORBENT-ASSAY; POLYSTYRENE SUPPORT; ELISA; HAPTEN; PLATES; HERBICIDES; BIOSENSOR; PROTEINS; SURFACE;

D O I：

10.1016/j.talanta.2013.05.012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A highly robust immunoassay applicable for the detection of aflatoxin B-1 (AFB(1)) using a Fab antibody fragment was developed. A key factor was the use of covalently immobilized AFB(1), which allowed an almost three fold increase in sensitivity, reduced assay time and regeneration with retention of binding capacity. Various factors that might affect the sensitivity of the assay such as pH, organic solvents, storage stability and wash stringency were critically evaluated. It was also demonstrated that the assay was applicable for determination of AFB(1), in corn samples at concentration within the European union regulatory limits. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：329 / 335

页数：7

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