Radioprotective Effects of Honeybee Venom (Apis mellifera) Against 915-MHz Microwave Radiation-Induced DNA Damage in Wistar Rat Lymphocytes: In Vitro Study

被引：52

作者：

Gajski, Goran ^{[1
]}

Garaj-Vrhovac, Vera ^{[1
]}

机构：

[1] Inst Med Res & Occupat Hlth, Mutagenesis Unit, Zagreb 10000, Croatia

来源：

INTERNATIONAL JOURNAL OF TOXICOLOGY | 2009年 / 28卷 / 02期

关键词：

bee venom; comet assay; formamidopyrimidine-DNA glycosylase (Fpg)-modified comet assay; microwave radiation; radioprotection; PERIPHERAL-BLOOD LYMPHOCYTES; VINYL-CHLORIDE MONOMER; BEE VENOM; CHROMOSOME-ABERRATIONS; ELECTROMAGNETIC-RADIATION; RADIOFREQUENCY RADIATION; CELL-PROLIFERATION; COMET ASSAY; 900; MHZ; EXPOSURE;

D O I：

10.1177/1091581809335051

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The aim of this study is to investigate the radioprotective effect of bee venom against DNA damage induced by 915-MHz microwave radiation (specific absorption rate of 0.6 W/kg) in Wistar rats. Whole blood lymphocytes of Wistar rats are treated with 1 mg/mL bee venom 4 hours prior to and immediately before irradiation. Standard and formamidopyrimidine-DNA glycosylase (Fpg)-modified comet assays are used to assess basal and oxidative DNA damage produced by reactive oxygen species. Bee venom shows a decrease in DNA damage compared with irradiated samples. Parameters of Fpg-modified comet assay are statistically different from controls, making this assay more sensitive and suggesting that oxidative stress is a possible mechanism of DNA damage induction. Bee venom is demonstrated to have a radioprotective effect against basal and oxidative DNA damage. Furthermore, bee venom is not genotoxic and does not produce oxidative damage in the low concentrations used in this study.

引用

页码：88 / 98

页数：11

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