An ultrasensitive rapid-response fluorescent probe for highly selective detection of HSA

被引：39

作者：

Li, Pengbo ^{[1
,2
]}

Wang, Yiru ^{[3
]}

Zhang, Shufang ^{[2
]}

Xu, Liang ^{[4
]}

Wang, Gongchen ^{[1
]}

Cui, Jingnan ^{[2
]}

机构：

[1] Dalian Sixth People Hosp, Dalian 116031, Peoples R China

[2] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116024, Peoples R China

[3] Dalian Med Univ, Dalian 116044, Peoples R China

[4] Dalian Univ Technol, Sch Chem, Dalian 116024, Peoples R China

来源：

TETRAHEDRON LETTERS | 2018年 / 59卷 / 14期

基金：

中国国家自然科学基金;

关键词：

Human serum albumin; Fluorescent probe; 2 '-Hydroxychalcone; TICT; Rapid detection; HUMAN-SERUM-ALBUMIN; DRUG BINDING-SITES; URINARY ALBUMIN; MICROALBUMINURIA; NEPHROPATHY; DEFICIENCY; PREDICTOR; COMPLEX;

D O I：

10.1016/j.tetlet.2018.02.065

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A fluorescent probe HCAB based on twisted intramolecular charge transfer (TICT) mechanism has been designed and synthesized by introducing benzoyl moiety to 4-dimethylamino-2'-hydroxychalcone. HCAB showed excellent selectivity towards human serum albumin (HSA) among different proteins with a remarkable 160-fold fluorescence enhancement and a wider linear range 0-100 mg/L of HSA. Job's Plot analysis suggested that the formation of HCAB-HSA complex followed a 1:1 stoichiometry. Molecular docking and the displacement assay demonstrated the binding site of HCAB was subdomain IIA and IB of HSA. We also tested HSA levels in human plasma for practical application, the results obtained from HCAB method were similar to those obtained from the standard clinical method. (C) 018 Elsevier Ltd. All rights reserved.

引用

页码：1390 / 1393

页数：4

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