Sensitive detection of near-infrared fluorescent dyes using high-performance liquid chromatography with peroxyoxalate chemiluminescence detection system

被引：0
|
作者：
Kimoto, K [1 ]
Gohda, R [1 ]
Murayama, K [1 ]
Santa, T [1 ]
Fukushima, T [1 ]
Homma, H [1 ]
Imai, K [1 ]
机构：
[1] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN
来源：
BIOMEDICAL CHROMATOGRAPHY | 1996年 / 10卷 / 04期
关键词：
D O I：
10.1002/(SICI)1099-0801(199607)10:4<189::AID-BMC585>3.0.CO;2-P
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The highly sensitive detection of four near-infrared (near-IR) fluorescent dyes, methylene blue, pyridine 1, oxazine 1 and 3,3'-diethylthiadicarbocyanine iodide (DTDCI) by high-performance liquid chromatography (HPLC) with post-column peroxyoxalate chemiluminescence (PO-CL) detection utilizing bis(4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl) oxalate (TDPO) and hydrogen peroxide was examined, The detection limits for methylene blue, pyridine 1, oxazine 1 and DTDCI were 120, 27, 31, 0.19 fmol on-column at a signal-to-noise ratio of 2, respectively, The sensitivity for DTDCI was 250 times that obtained by HPLC with the conventional fluorescent detection.
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页码：189 / 190
页数：2
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