Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR

被引：4

作者：

Suhaili, Zarizal ^{[1
]}

Johari, Saiful Azmi ^{[2
]}

Mohtar, Mastura ^{[2
]}

Abdullah, Ahmad Rushdi Tan ^{[3
]}

Ahmad, Affandi ^{[3
]}

Ali, Abdul Manaf ^{[1
]}

机构：

[1] Univ Darul Iman Malaysia UDM, Fac Agr & Biotechnol, Kuala Terengganu 20400, Terengganu, Malaysia

[2] Forest Res Inst Malaysia, Forest Biotechnol Div, Antimicrobial Lab, Med Plants Programme, Kepong 52109, Selangor, Malaysia

[3] Hosp Sultanah Nur Zahirah, Dept Pathol, Kuala Terengganu 20400, Terengganu, Malaysia

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2009年 / 25卷 / 02期

关键词：

Methicillin-resistant Staphylococcus aureus (MRSA); Nuc and mecA genes; Simplex and duplex real-time PCR; RAPID DETECTION; IDENTIFICATION; GENES; ASSAY; MECA;

D O I：

10.1007/s11274-008-9887-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The aim of this study was to develop a methicillin-resistant Staphylococcus aureus (MRSA) detection method based on the melting temperature analysis profiling of S. aureus clinical isolates from three different hospitals in Malaysia. Simplex and duplex real-time PCR assay was used for the simultaneous detection of nuc (species-specific) and mecA (methicillin-resistance) genes in a single SYBR Green I real-time PCR tube assay. Evaluations were based on the melting temperature (T (m)) analysis of the amplicons using 23 S. aureus clinical isolates including three ATCC S. aureus standard strains. Real-time PCR amplification products with melting peaks at 78.39 +/- A 0.4A degrees C and 74.41 +/- A 0.6A degrees C were detected for nuc and mecA genes, respectively. Each real-time PCR assay was completed within two hours. This rapid genotypic method is useful for the detection of resistant determinant (mecA) and identification of S. aureus (nuc) clinical isolates, thus benefiting patient therapy in hospitals.

引用

页码：253 / 258

页数：6

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