Detection of Malaysian methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates using simplex and duplex real-time PCR

被引：4
作者：
Suhaili, Zarizal [1 ]
Johari, Saiful Azmi [2 ]
Mohtar, Mastura [2 ]
Abdullah, Ahmad Rushdi Tan [3 ]
Ahmad, Affandi [3 ]
Ali, Abdul Manaf [1 ]
机构：
[1] Univ Darul Iman Malaysia UDM, Fac Agr & Biotechnol, Kuala Terengganu 20400, Terengganu, Malaysia
[2] Forest Res Inst Malaysia, Forest Biotechnol Div, Antimicrobial Lab, Med Plants Programme, Kepong 52109, Selangor, Malaysia
[3] Hosp Sultanah Nur Zahirah, Dept Pathol, Kuala Terengganu 20400, Terengganu, Malaysia
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2009年 / 25卷 / 02期
关键词：
Methicillin-resistant Staphylococcus aureus (MRSA); Nuc and mecA genes; Simplex and duplex real-time PCR; RAPID DETECTION; IDENTIFICATION; GENES; ASSAY; MECA;
D O I：
10.1007/s11274-008-9887-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aim of this study was to develop a methicillin-resistant Staphylococcus aureus (MRSA) detection method based on the melting temperature analysis profiling of S. aureus clinical isolates from three different hospitals in Malaysia. Simplex and duplex real-time PCR assay was used for the simultaneous detection of nuc (species-specific) and mecA (methicillin-resistance) genes in a single SYBR Green I real-time PCR tube assay. Evaluations were based on the melting temperature (T (m)) analysis of the amplicons using 23 S. aureus clinical isolates including three ATCC S. aureus standard strains. Real-time PCR amplification products with melting peaks at 78.39 +/- A 0.4A degrees C and 74.41 +/- A 0.6A degrees C were detected for nuc and mecA genes, respectively. Each real-time PCR assay was completed within two hours. This rapid genotypic method is useful for the detection of resistant determinant (mecA) and identification of S. aureus (nuc) clinical isolates, thus benefiting patient therapy in hospitals.
引用
收藏
页码：253 / 258
页数：6
相关论文
共 50 条
[21]   Genotypic and Phenotypic Characterization of Methicillin-Susceptible Staphylococcus aureus Isolates Misidentified as Methicillin-Resistant Staphylococcus aureus by the BD GeneOhm MRSA Assay [J].
Stamper, Paul D. ;
Louie, Lisa ;
Wong, Henry ;
Simor, Andrew E. ;
Farley, Jason E. ;
Carroll, Karen C. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2011, 49 (04) :1240-1244
[22]   Comparison of Chromogenic Media to BD GeneOhm Methicillin-Resistant Staphylococcus aureus (MRSA) PCR for Detection of MRSA in Nasal Swabs [J].
Bischof, Larry J. ;
Lapsley, Linda ;
Fontecchio, Karen ;
Jacosalem, Dollie ;
Young, Carol ;
Hankerd, Rosemary ;
Newton, Duane W. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (07) :2281-2283
[23]   Detection of Genes for Superantigen Toxins in Methicillin-Resistant Staphylococcus aureus Clinical Isolates in Karachi [J].
Taj, Yasmeen ;
Fatima, Izhar ;
Ali, Syed Wajid ;
Kazmi, Shahana U. .
JCPSP-JOURNAL OF THE COLLEGE OF PHYSICIANS AND SURGEONS PAKISTAN, 2014, 24 (02) :101-105
[24]   Comparison of Two Commercial PCR Methods for Methicillin-Resistant Staphylococcus aureus (MRSA) Screening in a Tertiary Care Hospital [J].
Aydiner, Aylin ;
Luesebrink, Jessica ;
Schildgen, Verena ;
Winterfeld, Ingo ;
Knuever, Oliver ;
Schwarz, Katja ;
Messler, Sabine ;
Schildgen, Oliver ;
Mattner, Frauke .
PLOS ONE, 2012, 7 (09)
[25]   Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) in drinking water fountains in urban parks [J].
Santos, Geyse A. C. ;
Dropa, Milena ;
Rocha, Solange M. ;
Peternella, Francisca A. S. ;
Razzolini, Maria Tereza Pepe .
JOURNAL OF WATER AND HEALTH, 2020, 18 (05) :654-664
[26]   Quantitative reverse transcription-PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus [J].
Wada, M. ;
Lkhagvadorj, E. ;
Bian, L. ;
Wang, C. ;
Chiba, Y. ;
Nagata, S. ;
Shimizu, T. ;
Yamashiro, Y. ;
Asahara, T. ;
Nomoto, K. .
JOURNAL OF APPLIED MICROBIOLOGY, 2010, 108 (03) :779-788
[27]   Evaluation of Methicillin-Resistant Staphylococcus aureus Carriage in the Elderly in Portugal Using Selective Enrichment Followed by quantitative real-time PCR [J].
Almeida, Sonia Tavares ;
Paulo, Ana Cristina ;
de Lencastre, Herminia ;
Sa-Leao, Raquel .
MICROBIAL DRUG RESISTANCE, 2022, 28 (05) :585-592
[28]   Drivers of methicillin-resistant Staphylococcus aureus (MRSA) lineage replacement in China [J].
Chen, Hongbin ;
Yin, Yuyao ;
van Dorp, Lucy ;
Shaw, Liam P. ;
Gao, Hua ;
Acman, Mislav ;
Yuan, Jizhen ;
Chen, Fengning ;
Sun, Shijun ;
Wang, Xiaojuan ;
Li, Shuguang ;
Zhang, Yawei ;
Farrer, Rhys A. ;
Wang, Hui ;
Balloux, Francois .
GENOME MEDICINE, 2021, 13 (01)
[29]   Recent Advances in the Detection of Methicillin Resistant Staphylococcus aureus (MRSA) [J].
Hulme, John .
BIOCHIP JOURNAL, 2017, 11 (02) :89-100
[30]   Real-time evaluation of an optimized real-time PCR assay versus Brilliance chromogenic MRSA agar for the detection of meticillin-resistant Staphylococcus aureus from clinical specimens [J].
Danial, J. ;
Noel, M. ;
Templeton, K. E. ;
Cameron, F. ;
Mathewson, F. ;
Smith, M. ;
Cepeda, J. A. .
JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (03) :323-328
12345