Mechanism of the Decay of Thymine Triplets in DNA Single Strands

被引：36

作者：

Pilles, Bert M. ^{[1
,2
]}

Bucher, Dominik B. ^{[1
,2
]}

Liu, Lizhe ^{[1
,2
]}

Clivio, Pascale ^{[4
]}

Gilch, Peter ^{[3
]}

Zinth, Wolfgang ^{[1
,2
]}

Schreier, Wolfgang J. ^{[1
,2
]}

机构：

[1] Univ Munich, Lehrstuhl BioMol Opt, Fak Phys, D-80538 Munich, Germany

[2] Univ Munich, Munich Ctr Integrated Prot Sci CIPSM, D-80538 Munich, Germany

[3] Univ Dusseldorf, Inst Phys Chem, D-40225 Dusseldorf, Germany

[4] Univ Reims, Inst Chim Mol Reims, UFR Pharm, CNRS,UMR 7312, F-51096 Reims, France

来源：

JOURNAL OF PHYSICAL CHEMISTRY LETTERS | 2014年 / 5卷 / 09期

关键词：

DIMER FORMATION; EXCITED-STATES; PHOTODIMERIZATION; DYNAMICS; URACIL; PHOTOCHEMISTRY; DIMERIZATION; PHOTODAMAGE; ABSORPTION; UNSTACKING;

D O I：

10.1021/jz500364g

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The decay of triplet states and the formation of cyclobutane pyrimidine dimers (CPDs) after UV excitation of the all-thymine oligomer (dT)(18) and the locked dinucleotide T(L)pT(L) were studied by nanosecond IR spectroscopy. IR marker bands characteristic for the CPD lesion and the triplet state were observed from similar to 1 ns (time resolution of the setup) onward. The amplitudes of the CPD marker bands remain constant throughout the time range covered (up to 10 mu s). The triplet decays with a time constant of similar to 10 ns presumably via a biradical intermediate (lifetime similar to 60 ns). This biradical has often been invoked as an intermediate for CPD formation via the triplet channel. The present results lend strong support to the existence of this intermediate, yet there is no indication that its decay contributes significantly to CPD formation.

引用

页码：1616 / 1622

页数：7

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