An ICT-based fluorescent probe guided by theoretical calculation for selectively mapping endogenous GSH in living cells

被引：20

作者：

Chen, Jiao ^{[1
]}

Li, Yiwei ^{[1
]}

Feng, Xukai ^{[1
]}

She, Mengyao ^{[1
,2
]}

Yao, Wenxin ^{[1
]}

Leng, Xin ^{[1
,2
]}

Liu, Ping ^{[1
]}

Zhang, Shengyong ^{[1
]}

Li, Jianli ^{[1
]}

机构：

[1] Northwest Univ, Key Lab Synthet & Nat Funct Mol, Minist Educ, Coll Chem & Mat Sci, Xian 710127, Shaanxi, Peoples R China

[2] Northwest Univ, Key Lab Resource Biol & Biotechnol Western China, Biomed Key Lab Shaanxi Prov,Minist Educ, Lab Tissue Engn,Coll Life Sci,Fac Life Sci & Med, Xian 710069, Shaanxi, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2021年 / 246卷

基金：

中国国家自然科学基金;

关键词：

Theoretical calculation; Fluorescent probe; ICT; Isophorone; Glutathione; Cells imaging; TURN-ON; GLUTATHIONE; CYSTEINE; DISCRIMINATION; STRATEGY; THERAPY; PRODRUG; CYS;

D O I：

10.1016/j.saa.2020.119041

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Glutathione (GSH) is one of the most essential bio-thiols to maintain the redox balance of organisms which is strongly associated with many physiological processes. Detecting the concentration and mapping the distribution of GSH in the living system is significant to study many related diseases. In this work, we have successfully constructed an ICT-based model to guide the design and synthesis of GSH specific fluorescent probe CF1. A serials spectroscopy test demonstrated that the response of CF1 towards GSH owned large stokes shift (similar to 167 nm) and an excellent linear relationship (0-120 mu M, R-2 = 0.9961). Furthermore, CF1 was successfully applied to image endogenous GSH in different cell lines with high sensitivity. This work is instructive for the oriented synthesis of ICT-based functional fluorescent probe and the further visualization of intracellular targets in the living system. (C) 2020 Elsevier B.V. All rights reserved.

引用

页数：7

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