The protective effect of protopanaxatriol-type saponin on intestinal health in antibiotic-treated mice

被引:1
作者
Zhang, Minxia [1 ,2 ,3 ]
Zhao, Jiaqi [4 ]
Deng, Jianjun [1 ,2 ,3 ]
Duan, Zhiguang [1 ,2 ,3 ]
Zhu, Chenhui [1 ,2 ,3 ]
Fan, Daidi [1 ,2 ,3 ]
机构
[1] Northwest Univ, Sch Chem Engn, Shaanxi Key Lab Degradable Biomed Mat, Xian 710069, Shaanxi, Peoples R China
[2] Northwest Univ, Sch Chem Engn, Shaanxi R&D Ctr Biomat & Fermentat Engn, Xian 710069, Shaanxi, Peoples R China
[3] Northwest Univ, Biotech & Biomed Res Inst, 229 North Taibai Rd, Xian 710069, Shaanxi, Peoples R China
[4] Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
INSULIN-RESISTANCE; GUT MICROBIOTA; EXPRESSION; ACTIVATION; OBESITY; PERMEABILITY; DIET;
D O I
10.1039/c9fo00242a
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Protopanaxatriol saponin (PPT) has excellent anti-cancer, anti-diabetes, and anti-anemia effects, but its effect on intestinal bacteria has rarely been studied. In this study, we investigated whether PPT has the ability to improve intestinal health in antibiotic-treated mice. Model mice were constructed using a broad-spectrum antibiotic, cephalosporin. The composition of the gut microbiota and relative concentration of short-chain fatty acids (SCFAs), short-chain fatty acid receptor proteins (GPR41, GPR43 and GPR109A), tight junction components (ZO-1 and occludin) and pro-inflammatory cytokines (TNF-alpha, IL-6, IL-1 beta, IL-22 and IFN-gamma) were determined. The results showed that PPT improved the composition of the gut microbiota, increased the concentration of SCFAs as well as receptor proteins and tight junction proteins, and decreased the pro-inflammatory cytokines. These findings indicate that PPT has a protective effect on intestinal microbes and enhances the integrity of the intestinal barrier as well as alleviates colonic inflammation in antibiotic-treated mice.
引用
收藏
页码:4124 / 4133
页数:10
相关论文
共 41 条
[1]   Treg induction by a rationally selected mixture of Clostridia strains from the human microbiota [J].
Atarashi, Koji ;
Tanoue, Takeshi ;
Oshima, Kenshiro ;
Suda, Wataru ;
Nagano, Yuji ;
Nishikawa, Hiroyoshi ;
Fukuda, Shinji ;
Saito, Takuro ;
Narushima, Seiko ;
Hase, Koji ;
Kim, Sangwan ;
Fritz, Joelle V. ;
Wilmes, Paul ;
Ueha, Satoshi ;
Matsushima, Kouji ;
Ohno, Hiroshi ;
Olle, Bernat ;
Sakaguchi, Shimon ;
Taniguchi, Tadatsugu ;
Morita, Hidetoshi ;
Hattori, Masahira ;
Honda, Kenya .
NATURE, 2013, 500 (7461) :232-+
[2]   Antibiotic-Induced Changes in the Intestinal Microbiota and Disease [J].
Becattini, Simone ;
Taur, Ying ;
Pamer, Eric G. .
TRENDS IN MOLECULAR MEDICINE, 2016, 22 (06) :458-478
[3]   Short, but Smart: SCFAs Train T Cells in the Gut to Fight Autoimmunity in the Brain [J].
Bhutia, Yangzom D. ;
Ganapathy, Vadivel .
IMMUNITY, 2015, 43 (04) :629-631
[4]   Profound Alterations of Intestinal Microbiota following a Single Dose of Clindamycin Results in Sustained Susceptibility to Clostridium difficile-Induced Colitis [J].
Buffie, Charlie G. ;
Jarchum, Irene ;
Equinda, Michele ;
Lipuma, Lauren ;
Gobourne, Asia ;
Viale, Agnes ;
Ubeda, Carles ;
Xavier, Joao ;
Pamer, Eric G. .
INFECTION AND IMMUNITY, 2012, 80 (01) :62-73
[5]   Local and systemic insulin resistance resulting from hepatic activation of IKK-β and NF-κB [J].
Cai, DS ;
Yuan, MS ;
Frantz, DF ;
Melendez, PA ;
Hansen, L ;
Lee, J ;
Shoelson, SE .
NATURE MEDICINE, 2005, 11 (02) :183-190
[6]   Metabolic endotoxemia initiates obesity and insulin resistance [J].
Cani, Patrice D. ;
Amar, Jacques ;
Iglesias, Miguel Angel ;
Poggi, Marjorie ;
Knauf, Claude ;
Bastelica, Delphine ;
Neyrinck, Audrey M. ;
Fava, Francesca ;
Tuohy, Kieran M. ;
Chabo, Chantal ;
Waget, Aurelie ;
Delmee, Evelyne ;
Cousin, Beatrice ;
Sulpice, Thierry ;
Chamontin, Bernard ;
Ferrieres, Jean ;
Tanti, Jean-Francois ;
Gibson, Glenn R. ;
Casteilla, Louis ;
Delzenne, Nathalie M. ;
Alessi, Marie Christine ;
Burcelin, Remy .
DIABETES, 2007, 56 (07) :1761-1772
[7]  
Carding Simon, 2015, Microbial Ecology in Health and Disease, V26, P26191, DOI 10.3402/mehd.v26.26191
[8]   Differential alteration in intestinal epithelial cell expression of Toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease [J].
Cario, E ;
Podolsky, DK .
INFECTION AND IMMUNITY, 2000, 68 (12) :7010-7017
[9]  
Chang C.J., 2017, NATURE COMMUNICATION, V8, P7489
[10]   Dietary saponins from four popular herbal tea exert prebiotic-like effects on gut microbiota in C57BL/6 mice [J].
Chen, Lei ;
Tai, William C. S. ;
Hsiao, W. L. Wendy .
JOURNAL OF FUNCTIONAL FOODS, 2015, 17 :892-902