Comparison of Heparin-Immobilized vs. Antibody-Immobilized Microspheres for the Capture and Detection of Cytokines during Microdialysis Sampling

被引：1

作者：

Duo, Jia ^{[1
]}

Cabrera, Randy F. Espinal ^{[2
]}

Stenken, Julie A. ^{[2
]}

机构：

[1] Rensselaer Polytech Inst, Dept Chem & Chem Biol, 110 8th St, Troy, NY 12180 USA

[2] Univ Arkansas, Dept Chem & Biochem, Fayetteville, AR 72701 USA

来源：

2009 IEEE/NIH LIFE SCIENCE SYSTEMS AND APPLICATIONS WORKSHOP | 2009年

关键词：

SURFACE;

D O I：

10.1109/LISSA.2009.4906722

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Heparin-immobilized beads were compared against antibody-immobilized beads for improving the collection of targeted cytokines through microdialysis sampling probes. Heparin offers an advantage over antibodies with respect to its stability and cost. The cytokines, MCP-1 and RANTES, exhibited increased flux through the probes when either heparin-immobilized or antibody-immobilized beads were included in the perfusion fluid. This suggests that heparin-immobilized beads may be a viable alternative to antibody-immobilized beads to increase the flux of select cytokines during microdialysis sampling.

引用

页码：112 / +

页数：2

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