Bioelectrochemical transformation of nicotinic acid into 6-hydroxynicotinic acid on Pseudomonas fluorescens TN5-immobilized column electrolytic flow system

Pseudomonas fluorescens TN5 catalyzes the hydroxylation of nicotinic acid (NA) into 6-hydroxynicotinic acid (6HNA), an important compound as a starting material for the synthesis of a new type of pesticides. Under aerobic conditions, however, 6HNA is metabolized in the P. fluorescens cells. The use of Fe(CN)(6)(3-) as an extracellular electron acceptor enhances the biotransformation of NA into 6HNA and completely suppresses the subsequent oxidation of 6HNA. The function of the P. fluorescens cell was combined with the electrode process by immobilizing the P. fluorescens cells on the carbon fiber electrode surface in the column, where Fe(CN)(6)(3-) was used as an electron transfer mediator. Continuous-flow electrolysis of NA in the presence of Fe(CN)(6)(3-) at the P. fluorescens-immobilized column electrode realized the accelerated and complete transformation of NA into 6HNA without any by-product. (C) 2000 Elsevier Science B.V. All rights reserved.