In vitro synthesis of cross-linked murein and its attachment to sacculi by PBP1A from Escherichia coli

被引：87

作者：

Born, Petra

Breukink, Eefjan

Vollmer, Waldemar

机构：

[1] Univ Tubingen, D-72076 Tubingen, Germany

[2] Univ Utrecht, Inst Biomembranes, Dept Biochem Membranes, Ctr Biomembranes & Lipid Enzymol, NL-3584 CH Utrecht, Netherlands

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2006年 / 281卷 / 37期

关键词：

D O I：

10.1074/jbc.M604083200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The penicillin-binding protein (PBP) 1A is a major murein (peptidoglycan) synthase in Escherichia coli. The murein synthesis activity of PBP1A was studied in vitro with radioactive lipid II substrate. PBP1A produced murein glycan strands by transglycosylation and formed peptide cross-links by transpeptidation. Time course experiments revealed that PBP1A, unlike PBP1B, required the presence of polymerized glycan strands carrying monomeric peptides for cross-linking activity. PBP1A was capable of attaching nascent murein synthesized from radioactive lipid II to nonlabeled murein sacculi. The attachment of the new material occurred by transpeptidation reactions in which monomeric tri- and tetrapeptides in the sacculi were the acceptors.

引用

页码：26985 / 26993

页数：9

共 33 条

[21] UDP-N-ACETYLMURAMYLPENTAPEPTIDE AS ACCEPTOR IN MUREIN BIOSYNTHESIS IN ESCHERICHIA-COLI MEMBRANES AND ETHER-PERMEABILIZED CELLS
KRAUS, W
GLAUNER, B
HOLTJE, JV
[J]. JOURNAL OF BACTERIOLOGY, 1985, 162 (03) : 1000 - 1004
[22] MACHINERY FOR CELL-GROWTH AND DIVISION - PENICILLIN-BINDING PROTEINS AND OTHER PROTEINS
MATSUHASHI, M
WACHI, M
ISHINO, F
[J]. RESEARCH IN MICROBIOLOGY, 1990, 141 (01) : 89 - 103
[23] NAKAGAWA J, 1984, J BIOL CHEM, V259, P3937
[24] MOLECULAR DIVERGENCE OF A MAJOR PEPTIDOGLYCAN SYNTHETASE WITH TRANSGLYCOSYLASE-TRANSPEPTIDASE ACTIVITIES IN ESCHERICHIA-COLI - PENICILLIN-BINDING PROTEIN 1BS
NAKAGAWA, J
MATSUHASHI, M
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 105 (04) : 1546 - 1553
[25] ANALYSIS OF THE DIFFERENT MOLECULAR-FORMS OF PENICILLIN-BINDING PROTEIN-1B IN ESCHERICHIA-COLI PONB MUTANTS LYSOGENIZED WITH SPECIALIZED TRANSDUCING-LAMBDA(PONB+) BACTERIOPHAGES
ROJO, F
AYALA, JA
DEPEDRO, MA
VAZQUEZ, D
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 144 (03): : 571 - 576
[26] Cloning and characterization of PBP1C, a third member of the multimodular class A penicillin-binding proteins of Escherichia coli
Schiffer, G
Höltje, JV
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (45) : 32031 - 32039
[27] MUTANTS OF ESCHERICHIA-COLI WHICH LACK A COMPONENT OF PENICILLIN-BINDING PROTEIN-1 ARE VIABLE
SPRATT, BG
JOBANPUTRA, V
SCHWARZ, U
[J]. FEBS LETTERS, 1977, 79 (02) : 374 - 378
[28] PROCESS OF CELLULAR DIVISION IN ESCHERICHIA-COLI - SERIES OF MUTANTS OF ESCHERICHIA-COLI ALTERED IN PENICILLIN-BINDING PROTEINS .11.
SUZUKI, H
NISHIMURA, Y
HIROTA, Y
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (02) : 664 - 668
[29] The catalytic, glycosyl transferase and acyl transferase modules of the cell wall peptidoglycan-polymerizing penicillin-binding protein 1b of Escherichia coli
Terrak, M
Ghosh, TK
van Heijenoort, J
Van Beeumen, J
Lampilas, M
Aszodi, J
Ayala, JA
Ghuysen, JM
Nguyen-Distèche, M
[J]. MOLECULAR MICROBIOLOGY, 1999, 34 (02) : 350 - 364
[30] FORMATION OF HYPER-CROSSLINKED PEPTIDOGLYCAN WITH MULTIPLE CROSSLINKAGES BY A PENICILLIN-BINDING PROTEIN, 1A, OF ESCHERICHIA-COLI
TOMIOKA, S
ISHINO, F
TAMAKI, S
MATSUHASHI, M
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 106 (04) : 1175 - 1182

← 1 2 3 4 →