Improved ethanol production from cheese whey, whey powder, and sugar beet molasses by "Vitreoscilla hemoglobin expressing" Escherichia coli

被引：18
|
作者：
Akbas, Meltem Yesilcimen [1 ]
Sar, Taner [1 ]
Ozcelik, Busra [1 ]
机构：
[1] Gebze Inst Technol, Dept Mol Biol & Genet, TR-41400 Gebze, Turkey
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2014年 / 78卷 / 04期
关键词：
bioethanol; Escherichia coli; sugar beet molasses; Vitreoscilla hemoglobin; whey; RECOMBINANT BURKHOLDERIA-CEPACIA; BIOETHANOL PRODUCTION; SACCHAROMYCES-CEREVISIAE; SWEET WHEY; FERMENTATION; BIODEGRADATION; 2-CHLOROBENZOATE; KINETICS; STRAINS; ENZYMES;
D O I：
10.1080/09168451.2014.896734
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This work investigated the improvement of ethanol production by engineered ethanologenic Escherichia coli to express the hemoglobin from the bacterium Vitreoscilla (VHb). Ethanologenic E. coli strain FBR5 and FBR5 transformed with the VHb gene in two constructs (strains TS3 and TS4) were grown in cheese whey (CW) medium at small and large scales, at both high and low aeration, or with whey powder (WP) or sugar beet molasses hydrolysate (SBMH) media at large scale and low aeration. Culture pH, cell growth, VHb levels, and ethanol production were evaluated after 48 h. VHb expression in TS3 and TS4 enhanced their ethanol production in CW (21-419%), in WP (17-362%), or in SBMH (48-118%) media. This work extends the findings that "VHb technology" may be useful for improving the production of ethanol from waste and byproducts of various sources.
引用
收藏
页码：687 / 694
页数：8
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