Role of bacteria in the adsorption and binding of DNA on soil colloids and minerals

被引：16
作者：
Cai, Peng [2 ]
Zhu, Jun [1 ]
Huang, Qiaoyun [1 ,2 ]
Fang, Linchun [1 ]
Liang, Wei [1 ]
Chen, Wenli [2 ]
机构：
[1] Huazhong Agr Univ, Minist Agr, Key Lab Subtrop Agr Resources & Environm, Wuhan 430070, Peoples R China
[2] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2009年 / 69卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Adsorption; Desorption; DNA; Bacteria; Composite; Soil colloid; Mineral; CLAY-MINERALS; BACILLUS-SUBTILIS; TRANSFORMATION; MONTMORILLONITE; PROTECTION; PROTEINS; SURFACES;
D O I：
10.1016/j.colsurfb.2008.10.008
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Adsorption and desorption of salmon sperm DNA on bacteria (Bacillus thuringiensis, Pseudomonas putida), two different colloidal fractions (organic and inorganic clay) from an Alfisol, minerals (montmorillonite, kaolinite and goethite) and colloid-bacteria composites were studied. Similar adsorption capacity and affinity of DNA were observed on two bacteria cells. However, the two bacteria strains played different roles in affecting the adsorption of DNA on the composites of soil colloidal particles with bacteria. The introduction of B. thuringiensis in soil colloids and minerals systems dramatically promoted DNA adsorption on colloidal particles especially organic clay, while P. putida decreased the adsorption of DNA on kaolinite and goethite. Electrostatic force and ligand exchange are regarded to be the major driving forces involved in the adsorption of DNA on bacterial cells, montmorillonite, soil colloids and goethite. Presence of bacteria enhanced the proportion of DNA adsorption on soil colloidal particles by electrostatic force and depressed that by ligand exchange process. Information obtained in this study is of fundamental significance for the understanding of the ultimate fate of extracellular DNA in soil systems. (c) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：26 / 30
页数：5
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