Exploring the expression of Pseudomonas aeruginosa genes directly from sputa of cystic fibrosis patients

被引：4

作者：

Barthe, C. ^{[1
]}

Nandakumar, S. ^{[1
,2
]}

Derlich, L. ^{[3
]}

Macey, J. ^{[3
]}

Bui, S. ^{[4
]}

Fayon, M. ^{[4
]}

Crouzet, M. ^{[1
]}

Garbay, B. ^{[1
,2
]}

Vilain, S. ^{[1
,2
]}

Costaglioli, P. ^{[1
,2
]}

机构：

[1] Univ Bordeaux, Biotechnol Prot Recombinantes Visee Sante, Bordeaux, France

[2] Bordeaux INP, Biotechnol Prot Recombinantes Visee Sante, F-33076 Bordeaux, France

[3] CHU Bordeaux, Serv Pneumol, CRCM Adulte, Pessac, France

[4] CHU Bordeaux, Ctr Invest Clin CIC 0005, Dept Pediat, CRCM Pediat, Bordeaux, France

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2015年 / 61卷 / 05期

关键词：

cystic fibrosis; gene expression; Pseudomonas aeruginosa; RT-qPCR; sputum; ALGINATE BIOSYNTHESIS; LUNG; TRANSCRIPTION; MIMICKING; MECHANISM; INFECTION; MUCOIDY; GROWTH; ALGR;

D O I：

10.1111/lam.12465

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Acquisition of Pseudomonas aeruginosa is known as a negative prognostic factor in patients with cystic fibrosis. We started a pilot study to evaluate Ps. aeruginosa gene expression directly from the sputum of infected patients. Total RNA was purified from 15 sputum samples collected from 10 patients, and the expression levels of five genes from Ps. aeruginosa were measured by RT-qPCR. Expression of algD, algR, antB, lasB and pqsA genes was determined in sputa that contained Ps. aeruginosa cells. The resultant data provided an overview of the expression of these genes in CF patients. Except for the correlation between algD expression and the mucoid phenotype, the gene expression profile could not be associated with the clinical status of patients. However, beyond the heterogeneity of the Ps. aeruginosa phenotype in sputum, we observed a correlation between the expression of antB and pqsA and a low level of lasB transcripts.

引用

页码：423 / 428

页数：6

共 28 条

[1] A quorum sensing-associated virulence gene of Pseudomonas aeruginosa encodes a LysR-like transcription regulator with a unique self-regulatory mechanism [J].

Cao, H ;

Krishnan, G ;

Goumnerov, B ;

Tsongalis, J ;

Tompkins, R ;

Rahme, LG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (25) :14613-14618

[2] Pseudomonas aeruginosa PqsA is an anthranilate-coenzyme a ligase [J].

Coleman, James P. ;

Hudson, L. Lynn ;

McKnight, Susan L. ;

Farrow, John M., III ;

Calfee, M. Worth ;

Lindsey, Claire A. ;

Pesci, Everett C. .

JOURNAL OF BACTERIOLOGY, 2008, 190 (04) :1247-1255

[3] Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients [J].

Costaglioli, Patricia ;

Barthe, Christophe ;

Fayon, Michael ;

Christoflour, Naik ;

Bui, Stephanie ;

Derlich, Laurence ;

Domblides, Philippe ;

Crouzet, Marc ;

Vilain, Sebastien ;

Garbay, Bertrand .

MOLECULAR AND CELLULAR PROBES, 2014, 28 (01) :10-12

[4] Evidence for the involvement of the anthranilate degradation pathway in Pseudomonas aeruginosa biofilm formation [J].

Costaglioli, Patricia ;

Barthe, Christophe ;

Claverol, Stephane ;

Broezel, Volker S. ;

Perrot, Michel ;

Crouzet, Marc ;

Bonneu, Marc ;

Garbay, Bertrand ;

Vilain, Sebastien .

MICROBIOLOGYOPEN, 2012, 1 (03) :326-339

[5] CLONING OF GENES-CONTROLLING ALGINATE BIOSYNTHESIS FROM A MUCOID CYSTIC-FIBROSIS ISOLATE OF PSEUDOMONAS-AERUGINOSA [J].

DARZINS, A ;

CHAKRABARTY, AM .

JOURNAL OF BACTERIOLOGY, 1984, 159 (01) :9-18

[6] Role of the Pseudomonas aeruginosa las and rhl quorum-sensing systems in rhlI regulation [J].

de Kievit, TR ;

Kakai, Y ;

Pesci, EC ;

Iglewski, BH .

FEMS MICROBIOLOGY LETTERS, 2002, 212 (01) :101-106

[7] GENE ALGD CODING FOR GDPMANNOSE DEHYDROGENASE IS TRANSCRIPTIONALLY ACTIVATED IN MUCOID PSEUDOMONAS-AERUGINOSA [J].

DERETIC, V ;

GILL, JF ;

CHAKRABARTY, AM .

JOURNAL OF BACTERIOLOGY, 1987, 169 (01) :351-358

[8] CONTROL OF MUCOIDY IN PSEUDOMONAS-AERUGINOSA - TRANSCRIPTIONAL REGULATION OF ALGR AND IDENTIFICATION OF THE 2ND REGULATORY GENE, ALGQ [J].

DERETIC, V ;

KONYECSNI, WM .

JOURNAL OF BACTERIOLOGY, 1989, 171 (07) :3680-3688

[9] Gene expression of Pseudomonas aeruginosa in a mucin-containing synthetic growth medium mimicking cystic fibrosis lung sputum [J].

Fung, Carina ;

Naughton, Sharna ;

Turnbull, Lynne ;

Tingpej, Pholawat ;

Rose, Barbara ;

Arthur, Jonathan ;

Hu, Honghua ;

Harmer, Christopher ;

Harbour, Colin ;

Hassett, Daniel J. ;

Whitchurch, Cynthia B. ;

Manos, Jim .

JOURNAL OF MEDICAL MICROBIOLOGY, 2010, 59 (09) :1089-1100

[10] Pathophysiology and management of pulmonary infections in cystic fibrosis [J].

Gibson, RL ;

Burns, JL ;

Ramsey, BW .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2003, 168 (08) :918-951

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