Experimental measurement of the strength of a Cα-H•••O bond in a lipid bilayer

被引：104

作者：

Arbely, E ^{[1
]}

Arkin, IT ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Dept Biol Chem, Alexander Silberman Inst Life Sci, IL-91904 Jerusalem, Israel

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 17期

关键词：

D O I：

10.1021/ja049826h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Due to the apolar nature of the lipid bilayer, the weak Cα-H···O H-bond is thought to contribute significantly toward the stability of transmembrane helical bundles such as glycophorin A (GPA). Here for the first time we measured the strength of such a bond, using vibrational frequency shifts of a dimeric and nondimeric variants of GPA containing a Gly CD2 label. Although the resulting estimated bond strength of 0.88 kcal/mol is relatively weak, several such bonds could contribute significantly toward bundle stabilization. Copyright © 2003 American Chemical Society.

引用

页码：5362 / 5363

页数：2

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