Analysis of minichromosome maintenance proteins as a novel method for detection of colorectal cancer in stool

被引：137

作者：

Davies, RJ

Freeman, A

Morris, LS

Bingham, S

Dilworth, S

Scott, I

Laskey, RA

Miller, R

Coleman, N ^{[1
]}

机构：

[1] MRC, Canc Cell Univ, Hutchison MRC Res Ctr, Cambridge CB2 2XZ, England

[2] Addenbrookes NHS Trust, Dept Surg, Cambridge CB2 2QQ, England

[3] MRC, Dunn Human Nutr Unit, Cambridge, England

[4] Hammersmith Hosp, Imperial Coll Sch Med, Dept Metab Med, London, England

来源：

LANCET | 2002年 / 359卷 / 9321期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/S0140-6736(02)08739-1

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Colorectal cancer Is a common disease, and more reliable screening methods are needed for early detection. We aim to develop a non-Invasive, stool-based assay that can identify colorectal cancer by detection of minichromosome maintenance protein 2 (MCM2) expression in colonocytes retrieved from the faecal surface. We devised a cell line model to investigate methods and conditions for optimum colonocyte retrieval. In our clinical evaluation study, MCM2-positive cells were retrieved from 37 of 40 patients with symptomatic colorectal cancer, but from none of 25 healthy control Individuals. These results suggest that immunocytochemical analysis of retrieved colonocytes might enable accurate detection of colorectal cancer in stool.

引用

页码：1917 / 1919

页数：3

共 5 条

[1]

Freeman A, 1999, CLIN CANCER RES, V5, P2121

[2]

Loktionov A, 1998, CLIN CANCER RES, V4, P337

[3] Biologic factors and response to radiotherapy in carcinoma of the cervix [J].

Mukherjee, G ;

Freeman, A ;

Moore, R ;

Kumaraswamy ;

Devi, KU ;

Morris, LS ;

Coleman, N ;

Dilworth, S ;

Prabhakaran, PS ;

Stanley, MA .

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2001, 11 (03) :187-193

[4] Detection of APC mutations in fecal DNA from patients with colorectal tumors [J].

Traverso, G ;

Shuber, A ;

Levin, B ;

Johnson, C ;

Olsson, L ;

Schoetz, DJ ;

Hamilton, SR ;

Boynton, K ;

Kinzler, KW ;

Vogelstein, B .

NEW ENGLAND JOURNAL OF MEDICINE, 2002, 346 (05) :311-320

[5] Improved cervical smear assessment using antibodies against proteins that regulate DNA replication [J].

Williams, GH ;

Romanowski, P ;

Morris, L ;

Madine, M ;

Mills, AD ;

Stoeber, K ;

Marr, J ;

Laskey, RA ;

Coleman, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14932-14937

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