Removal of 2,4-dichlorophenol by chitosan-immobilized laccase from Coriolus versicolor

被引：81
作者：
Zhang, Jianbo [1 ,2 ]
Xu, Zhenqiang [1 ,2 ]
Chen, Hui [1 ,2 ]
Zong, Yueru [1 ,2 ]
机构：
[1] Peking Univ, Coll Environm Sci & Engn, Dept Environm Sci, Beijing 100871, Peoples R China
[2] Peking Univ, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100871, Peoples R China
来源：
BIOCHEMICAL ENGINEERING JOURNAL | 2009年 / 45卷 / 01期
关键词：
Immobilization; Enzyme activity; Catalysis; Optimum condition; LENTINULA-EDODES; ELIMINATION; FUNGI;
D O I：
10.1016/j.bej.2009.02.005
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Laccase from Coriolus versicolor was immobilized on chitosan using glutaraldehyde as a cross-linking agent. After immobilization, laccase retained 52.2% of its original activity and was used to study 2,4-dichlorophenol (2,4-DCP) removal from aqueous solutions. The optimum pH for 2,4-DCP removal by the immobilized laccase was similar to 5.5, which was lower than the optimal pH of 6.0 for free laccase catalysis. Immobilized laccase also had an atypically wide optimum temperature range for catalysis of 35-45 degrees C. Immobilized laccase could be used repeatedly, and its removal efficiency for 2,4-DCP remained above similar to 50% for up to six usages. (c) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：54 / 59
页数：6
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