Development of a mass-directed preparative supercritical fluid chromatography purification system

被引：24
作者：
Zhang, Xu [1 ]
Towle, Marc H. [1 ]
Felice, Christine E. [1 ]
Flament, James H. [1 ]
Goetzinger, Wolfgang K. [1 ]
机构：
[1] ArQule Inc, Woburn, MA 01801 USA
来源：
JOURNAL OF COMBINATORIAL CHEMISTRY | 2006年 / 8卷 / 05期
关键词：
D O I：
10.1021/cc0600674
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
In this paper, we report the development of a mass-directed supercritical fluid chromatography (SFC) purification system. We have addressed issues on software compatibility, the interface between the preparative SFC and the mass spectrometer, and fraction collection. Good peak shape and signal were achieved in the mass spectrometry (MS) trace, allowing accurate peak detection and reliable fraction collection. Simple modifications on a commercially available fraction collector enabled fractionation at atmospheric pressure with high recovery. The SFC/MS purification system has been used in support of high-throughput library purification and has been proven to be a valuable tool in complementing our reversed-phase high- performance liquid chromatograph (RP-HPLC/MS)-based technology platform.
引用
收藏
页码：705 / 714
页数：10
相关论文
共 42 条
  • [1] Preparative high-performance liquid chromatography-mass spectrometry for the high-throughput purification of combinatorial libraries
    Bauser, M
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2002, 40 (05) : 292 - 296
  • [2] COMPOSITION AND DENSITY EFFECTS USING METHANOL CARBON-DIOXIDE IN PACKED-COLUMN SUPERCRITICAL FLUID CHROMATOGRAPHY
    BERGER, TA
    DEYE, JF
    [J]. ANALYTICAL CHEMISTRY, 1990, 62 (11) : 1181 - 1185
  • [3] High-speed screening of combinatorial libraries by gradient packed-column supercritical fluid chromatography
    Berger, TA
    Wilson, WH
    [J]. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2000, 43 (1-3): : 77 - 85
  • [4] BERGER TA, 2004, Patent No. 6685828
  • [5] SFC/MS in drug discovery at Pfizer, La Jolla
    Bolaños, B
    Greig, M
    Ventura, M
    Farrell, W
    Aurigemma, CM
    Li, H
    Quenzer, TL
    Tivel, K
    Bylund, JMR
    Tran, P
    Pham, C
    Phillipson, D
    [J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2004, 238 (02) : 85 - 97
  • [6] CALDWELL J, 2004, P METTL TOL AUTOCHEM
  • [7] An automated LC method for the small-scale purification of organic molecules derived from combinatorial libraries
    Chan, ECY
    Wang, AW
    Jia, WP
    Dollinger, GD
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2002, 29 (1-2) : 139 - 146
  • [8] Pressure-regulating fluid interface and phase behavior considerations in the coupling of packed-column supercritical fluid chromatography with low-pressure detectors
    Chester, TL
    Pinkston, JD
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1998, 807 (02) : 265 - 273
  • [9] Parallel preparative high-performance liquid chromatography with on-line molecular mass characterization
    Edwards, C
    Liu, JB
    Smith, TJ
    Brooke, D
    Hunter, DJ
    Organ, A
    Coffey, P
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003, 17 (18) : 2027 - 2033
  • [10] High throughput HPLC/MS purification in support of drug discovery
    Goetzinger, W
    Zhang, X
    Bi, G
    Towle, M
    Cherrak, D
    Kyranos, JN
    [J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2004, 238 (02) : 153 - 162
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