Effect of alkylglycerone phosphate synthase on the expression profile of circRNAs in the human thyroid cancer cell line FRO

被引：15

作者：

Hou, Shasha ^{[1
,2
]}

Tan, Jian ^{[1
]}

Yang, Bing ^{[3
]}

He, Lu ^{[4
]}

Zhu, Yu ^{[5
]}

机构：

[1] Tianjin Med Univ, Gen Hosp, Dept Nucl Med, Tianjin 300052, Peoples R China

[2] Tianjin Med Univ, Sch Basic Med Sci, Grad Sch, Tianjin 300070, Peoples R China

[3] Tianjin Med Univ, Sch Basic Med Sci, Dept Cell Biol, Tianjin 300070, Peoples R China

[4] Tianjin Med Univ, Sch Basic Med Sci, Dept Anat & Histol, 22 Qixiangtai Rd, Tianjin 300070, Peoples R China

[5] Tianjin Huanhu Hosp, Tianjin Key Lab Cerebral Vessels & Neurodegenerat, Dept Clin Lab, 6 Jizhao Rd, Tianjin 300350, Peoples R China

来源：

ONCOLOGY LETTERS | 2018年 / 15卷 / 05期

基金：

中国国家自然科学基金;

关键词：

circular RNAs; thyroid cancer; alkylglycerone phosphate synthase; CARCINOMA; PROLIFERATION; PROGRESSION; PHENOTYPE; GLIOMA; AGPS;

D O I：

10.3892/ol.2018.8356

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Thyroid cancer is a common primary tumor in China. Therefore, it is important to investigate the underlying molecular mechanism of thyroid cancer in order to achieve effective individualized treatments. In our previous study, a positive correlation between the expression of alkylglycerone phosphate synthase (AGPS) and the malignant phenotype of thyroid cancer cell lines was identified. The inactivation of AGPS was able to decrease the malignancy of cancer, and inhibit tumor growth and invasion. However, the function of AGPS on thyroid cancer was unclear. In the present study, it was revealed that AGPS was able to regulate the expression of circular RNAs (circRNAs), which may be the mechanism of its anticancer activity. Therefore, the effects of AGPS silencing and knockout on circRNA expression in the thyroid cancer cell line FRO were investigated using circRNAs microarray, and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed in order to investigate the underlying molecular mechanism of AGPS for the regulation of thyroid cancer through circRNAs.

引用

页码：7889 / 7899

页数：11

共 21 条

[11] Screening of the prognostic targets for breast cancer based co-expression modules analysis [J].

Liu, Huijuan ;

Ye, Hui .

MOLECULAR MEDICINE REPORTS, 2017, 16 (04) :4038-4044

[12]

Liu JH, 2017, SPRING SER ASIA CRIM, P1, DOI 10.1007/978-3-319-54942-2_1

[13] Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].

Livak, KJ ;

Schmittgen, TD .

METHODS, 2001, 25 (04) :402-408

[14] Microarray profiling of circular RNAs in human papillary thyroid carcinoma [J].

Peng, Nianchun ;

Shi, Lixin ;

Zhang, Qiao ;

Hu, Ying ;

Wang, Nanpeng ;

Ye, Hui .

PLOS ONE, 2017, 12 (03)

[15] Discovery of Inhibitors for the Ether Lipid-Generating Enzyme AGPS as Anti-Cancer Agents [J].

Piano, Valentina ;

Benjamin, Daniel I. ;

Valente, Sergio ;

Nenci, Simone ;

Marrocco, Biagina ;

Mai, Antonello ;

Aliverti, Alessandro ;

Nomura, Daniel K. ;

Mattevi, Andrea .

ACS CHEMICAL BIOLOGY, 2015, 10 (11) :2589-2597

[16] limma powers differential expression analyses for RNA-sequencing and microarray studies [J].

Ritchie, Matthew E. ;

Phipson, Belinda ;

Wu, Di ;

Hu, Yifang ;

Law, Charity W. ;

Shi, Wei ;

Smyth, Gordon K. .

NUCLEIC ACIDS RESEARCH, 2015, 43 (07) :e47

[17] The Lncrna-TUG1/EZH2 Axis Promotes Pancreatic Cancer Cell Proliferation, Migration and EMT Phenotype Formation Through Sponging Mir-382 [J].

Zhao, Liang ;

Sun, Hongwei ;

Kong, Hongru ;

Chen, Zongjing ;

Chen, Bicheng ;

Zhou, Mengtao .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2017, 42 (06) :2145-2158

[18] Estimates of cancer incidence and mortality in China, 2013 [J].

Zheng, Rongshou ;

Zeng, Hongmei ;

Zhang, Siwei ;

Chen, Wanqing .

CHINESE JOURNAL OF CANCER, 2017, 36

[19] Natural Killer Cell (NK-92MI)-Based Therapy for Pulmonary Metastasis of Anaplastic Thyroid Cancer in a Nude Mouse Model [J].

Zhu, Liya ;

Li, Xiu Juan ;

Kalimuthu, Senthilkumar ;

Gangadaran, Prakash ;

Lee, Ho Won ;

Oh, Ji Min ;

Baek, Se Hwan ;

Jeong, Shin Young ;

Lee, Sang-Woo ;

Lee, Jaetae ;

Ahn, Byeong-Cheol .

FRONTIERS IN IMMUNOLOGY, 2017, 8

[20] Role and mechanism of the alkylglycerone phosphate synthase in suppressing the invasion potential of human glioma and hepatic carcinoma cells in vitro [J].

Zhu, Yu ;

Zhu, Lielie ;

Lu, Ling ;

Zhang, Ling ;

Zhang, Guodong ;

Wang, Qin ;

Yang, Ping .

ONCOLOGY REPORTS, 2014, 32 (01) :431-436

← 1 2 3 →