Growth arrest and decrease of α-SMA and type I collagen expression by palmitic acid in the rat hepatic stellate cell line PAV-1

被引:38
作者
Abergel, Armand
Sapin, Vincent
Dif, Nicolas
Chassard, Christophe
Darcha, Claude
Marcand-Sauvant, Julie
Gaillard-Martinie, Brigitte
Rock, Edmond
Dechelotte, Pierre
Sauvant, Patrick
机构
[1] INRA Clermont Fd Theix, Equipe Vitamines, Unite Maladies Metab & Micronutr, F-63122 St Genes Champanelle, France
[2] Ctr Hosp Univ, Hotel Dieu, Ctr Hepato Gastroenterol, F-63058 Clermont Ferrand, France
[3] INSERM, UMR 384, Fac Med, Clermont Ferrand, France
[4] INRA Clermont Fd Theix, Lab Microbiol, F-63122 St Genes Champanelle, France
关键词
vitamin A; palmitic acid; cell growth; hepatic stellate cell;
D O I
10.1007/s10620-005-9031-y
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Liver fibrosis is characterized by an activation of hepatic stellate cells (HSC). During primary culture HSC evolve from a quiescent into an activated phenotype which is characterized by alpha-smooth muscle actin (alpha-SMA) up-regulation, increase in cell growth, and extracellular matrix secretion. HSC culture with trans-resveratrol can lead to deactivation of myofibroblast-like HSC. We used an HSC line, PAV-1, to check the role of retinol and palmitic acid in the deactivation process of HSC. Using mass and metabolic-based methods, Western blot and immunocytochemistry assays, we demonstrated that treatment with palmitic acid (75 mu M) alone or in combination with retinol (2 mu M) significantly decreased cell proliferation and alpha-SMA expression. We also established that the association of both compounds strongly decreased collagen type I expression. Our results suggest the potential use of palmitic acid alone or in combination with retinol to induce HSC deactivation.
引用
收藏
页码:986 / 995
页数:10
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