The Ca2+-activated K+ current of human sperm is mediated by Slo3

被引：97

作者：

Brenker, Christoph ^{[1
]}

Zhou, Yu ^{[2
]}

Mueller, Astrid ^{[1
]}

Echeverry, Fabio Andres ^{[1
]}

Troetschel, Christian ^{[3
]}

Poetsch, Ansgar ^{[3
]}

Xia, Xiao-Ming ^{[2
]}

Boenigk, Wolfgang ^{[1
]}

Lingle, Christopher J. ^{[2
]}

Kaupp, U. Benjamin ^{[1
]}

Struenker, Timo ^{[1
]}

机构：

[1] Ctr Adv European Studies & Res, Dept Mol Sensory Syst, Bonn, Germany

[2] Washington Univ, Sch Med, Dept Anesthesiol, St Louis, MO 63110 USA

[3] Ruhr Univ Bochum, Lehrstuhl Biochem Pflanzen, Bochum, Germany

来源：

ELIFE | 2014年 / 3卷

基金：

美国国家卫生研究院;

关键词：

MAMMALIAN SPERMATOZOA; INTRACELLULAR CA2+; RAPID EVOLUTION; CATSPER CHANNEL; BK CHANNELS; PROGESTERONE; VOLTAGE; SENSITIVITY; ACTIVATION; INFLUX;

D O I：

10.7554/eLife.01438

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sperm are equipped with a unique set of ion channels that orchestrate fertilization. In mouse sperm, the principal K+ current (I-KSper) is carried by the Slo3 channel, which sets the membrane potential (V-m) in a strongly pH(i)-dependent manner. Here, we show that I-KSper in human sperm is activated weakly by pH(i) and more strongly by Ca2+. Correspondingly, V-m is strongly regulated by Ca2+ and less so by pH(i). We find that inhibitors of Slo3 suppress human I-KSper, and we identify the Slo3 protein in the flagellum of human sperm. Moreover, heterologously expressed human Slo3, but not mouse Slo3, is activated by Ca2+ rather than by alkaline pH(i); current-voltage relations of human Slo3 and human IKSper are similar. We conclude that Slo3 represents the principal K+ channel in human sperm that carries the Ca2+-activated I-KSper current. We propose that, in human sperm, the progesterone-evoked Ca2+ influx carried by voltage-gated CatSper channels is limited by Ca2+-controlled hyperpolarization via Slo3.

引用

页数：19

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