Optimization of culture conditions for gamma-aminobutyric acid production by newly identified Pediococcus pentosaceus MN12 isolated from 'mam nem', a fermented fish sauce

被引：18

作者：

Do Thi Bich Thuy ^{[1
]}

AnTien Nguyen ^{[2
]}

Khoo, Kuan Shiong ^{[3
]}

Chew, Kit Wayne ^{[4
]}

Cnockaert, Margo ^{[5
]}

Vandamme, Peter ^{[5
]}

Ho, Yeek-Chia ^{[6
,7
]}

Nguyen Duc Huy ^{[8
]}

Cocoletzi, Heriberto Hernandez ^{[9
]}

Show, Pau Loke ^{[3
]}

机构：

[1] Hue Univ, Hue Univ Agr & Forestry, Hue, Vietnam

[2] Dalat Univ, Fac Agr & Forestry, Da Lat, Vietnam

[3] Univ Nottingham Malaysia, Fac Sci & Engn, Dept Chem & Environm Engn, Selangor Darul Ehsan, Malaysia

[4] Xiamen Univ Malaysia, Sch Energy & Chem Engn, Jalan Sunsuria, Sepang, Selangor, Malaysia

[5] Univ Ghent, Dept Biochem & Microbiol, Lab Microbiol, Ghent, Belgium

[6] Univ Teknol Petronas, Civil & Environm Engn Dept, Seri Iskandar, Perak, Malaysia

[7] Univ Teknol Petronas, Inst Self Sustainable Bldg, Ctr Urban Resource Sustainabil, Perak Darul Ridzuan, Malaysia

[8] Hue Univ, Inst Biotechnol, Hue, Vietnam

[9] Benemerita Univ Autonoma Puebla, Fac Ingn Quim, Puebla, Mexico

来源：

BIOENGINEERED | 2021年 / 12卷 / 01期

关键词：

GABA; optimization; Pedioccocus pentosaceus; fermented fish sauce; CORYNEBACTERIUM-GLUTAMICUM; GABA; BACTERIA; DISEASE; KIMCHI; BLACK; FOODS;

D O I：

10.1080/21655979.2020.1857626

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study was aimed to identify and optimize the culture conditions for gamma-aminobutyric acid (GABA) production by a lactic acid bacterium strain isolated from mam nem, a fermented fish sauce. Among the six isolates obtained from mam nem, the MN12 had the most potent GABA-producing capability. The strain was then identified to be Pedioccocus pentosaceus by employing MALDI-TOF-MS and phenylalanyl-tRNA synthase sequencing methods. The initial cell density of 5.10(6) CFU/mL, monosodium glutamate concentration of 60 mM, initial pH of 7, temperature of 45 degrees C and cultivation time of 72 h were found to be the optimal culture conditions for highest production of GABA, reaching 27.9 +/- 0.42 mM, by this strain. The cultivation conditions for GABA production by P. pentosaceus MN12 have been successfully optimized, providing a foundation for the development of fermented foods enriched with GABA.

引用

页码：54 / 62

页数：9

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