Laboratory on chip for clinical applications

For fast and continuous measurements of metabolic parameters small and easy-to-handle analytic devices with integrated chemo- and biosensors implemented in a fluidic microsystem were developed. Such microsystem was chosen for minimal invasive measurement, to overcome analyte handling problems and to perform continuous measurements. Integrated sensors for measuring oxygen, CO2, glucose, lactate, glutamine, glutamate concentrations and enzyme activities were produced using microelectronic technology and electrochemical detection principles. Different enzymes were immobilized in photopatterned pHEMA layers and used as biosensor array. A microfluidic allows continuous steady state measurements as well as performing flow protocols for the determination of enzyme activities.