Immobilized Jerusalem artichoke (Helianthus tuberosus) tissue electrode for phenol detection

被引：7
|
作者：
Odaci, D [1 ]
Timur, S [1 ]
Telefoncu, A [1 ]
机构：
[1] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey
来源：
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2004年 / 32卷 / 02期
关键词：
tissue based biosensor; phenol; Jerusalem artichoke; (Helianthus tuberosus);
D O I：
10.1081/BIO-120037836
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A tissue based biosensor for the determination of phenol was developed by using Jerusalem artichoke (Helianthus tuberosus) in combination with a dissolved oxygen (DO) probe. The tissue electrode response depends linearly on phenol concentration between 0.002 and 0.010 muM in 10 min response time. Maximum electrode response was found in phosphate buffer at pH 8.0 and 35 degreesC. The reproducibility of the enzyme electrode was also tested by using standard phenol solutions (0.005 muM). The standard deviation (SD) and variation coefficient (cv) were calculated as +/-1.4 x 10(-4) muM and 3.1%, respectively.
引用
收藏
页码：315 / 323
页数：9
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