Algoriphagus aestuarii sp nov., a member of the Cyclobacteriaceae isolated from a tidal-flat sediment of the Yellow Sea in Korea

A Gram-strain-negative, coccoid or oval-shaped, non-motile bacterial strain, designated MDM-1(T), Was isolated from a tidal-flat sediment on the Korean peninsula. Strain MDM-1(T) was found to grow optimally at pH 7.0-8.0, at 30 degrees C and in the presence of 2-3 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain MDM-1(T) falls within the clade comprising species of the genus Algoriphagus, clustering with the type strains of Algoriphagus halophilus, A. lutimaris, A. chungangensis and A. machipongonensis, with which it exhibited 97.2-98.5 % 16S rRNA gene sequence similarity. Sequence similarities to the type strains of the other recognized species of the genus Algoriphagus were 92.8-97.6 %. Strain MDM-1(T) was found to contain MK-7 as the predominant menaquinone and iso-C-15:0 and summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c) as the major fatty acids. The major polar lipids were identified as phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain MDM-1(T) Was determined to be 42.7 mol% and the mean DNA DNA relatedness with A. halophilus KCTC 12051(T), A. lutimaris S1-3(T), A. chungangensis KCTC 23759(T), A. machipongonensis DSM 24695(T) and A. ratkowskyi ClP 107452(T) was 19.7-5.2 %. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain MDM-1(T) is distinguishable from recognized species of the genus Algoriphagus. On the basis of the data presented, strain MDM-1(T) IS proposed to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus aestuarii sp. nov. is proposed. The type strain is MDM-1(T) (= KCTC 42199(T)=NBRC 110552(T)).