y A MAPK cascade downstream of IDA-HAE/HSL2 ligand-receptor pair in lateral root emergence

被引：123

作者：

Zhu, Qiankun ^{[1
,4
]}

Shao, Yiming ^{[1
]}

Ge, Shating ^{[1
]}

Zhang, Mengmeng ^{[1
]}

Zhang, Tianshu ^{[1
]}

Hu, Xiaotian ^{[1
]}

Liu, Yidong ^{[2
]}

Walker, John ^{[3
]}

Zhang, Shuqun ^{[2
]}

Xu, Juan ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou, Zhejiang, Peoples R China

[2] Univ Missouri, Div Biochem, Interdisciplinary Plant Grp, Bond Life Sci Ctr, Columbia, MO 65211 USA

[3] Univ Missouri, Div Biol Sci, Interdisciplinary Plant Grp, Columbia, MO 65211 USA

[4] Nanjing Agr Univ, Coll Plant Protect, Dept Plant Pathol, Nanjing, Jiangsu, Peoples R China

来源：

NATURE PLANTS | 2019年 / 5卷 / 04期

基金：

中国国家自然科学基金;

关键词：

ACTIVATED PROTEIN-KINASE; FLORAL ORGAN ABSCISSION; CELL-SEPARATION; STOMATAL DEVELOPMENT; ARABIDOPSIS; MPK6; INFLORESCENCE; BIOSYNTHESIS; ARCHITECTURE; MECHANISMS;

D O I：

10.1038/s41477-019-0396-x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Lateral root (LR) emergence is a highly coordinated process involving precise cell-cell communication. Here, we show that MITOGEN-ACTIVATED PROTEIN KINASE3 (MPK3) and MPK6, and their upstream MAP-kinase kinases (MAPKKs), MKK4 and MKK5, function downstream of HAESA (HAE)/HAESA-LIKE2 (HSL2) and their ligand INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) during LR emergence. Loss of function of MKK4/MKK5 or MPK3/MPK6 results in restricted passage of the growing lateral root primordia (LRP) through the overlaying endodermal, cortical and epidermal cell layers, leading to reduced LR density. The MKK4/MKK5-MPK3/MPK6 module regulates the expression of cell wall remodelling genes in cells overlaying LRP and therefore controls pectin degradation in the middle lamella. Expression of constitutively active MKK4 or MKK5 driven by the HAE or HSL2 promoter fully rescues the LR emergence defect in the ida and hae hsl2 mutants. In addition, the MKK4/MKK5-MPK3/MPK6 module is indispensable in auxin-facilitated LR emergence. Our study provides insights into the auxin-governed and IDA-HAE/HLS2 ligand-receptor pair-mediated LR emergence process.

引用

页码：414 / 423

页数：10

共 46 条

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