Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes

被引：1

作者：

Xu, Y. -J. ^{[1
]}

Liao, M. -J. ^{[1
]}

Wang, Y. -G. ^{[1
]}

Liu, Z. -C. ^{[1
]}

Qin, P. ^{[1
]}

Zhang, Z. ^{[1
]}

Rong, X. -J. ^{[1
]}

Liu, X. -Z. ^{[1
]}

机构：

[1] Chinese Acad Fishery Sci, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2013年 / 12卷 / 04期

关键词：

Paralichthys lethostigma; Microsatellite marker; Cross-species amplification; Pleuronectiformes; SOUTHERN FLOUNDER; BROODSTOCK;

D O I：

10.4238/2013.December.16.2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the genetic diversity of the southern flounder Parctlichthys lethostigina. Microsatellite-enriched libraries were constructed and novel microsatellite markers were developed and applied for genetic detection of wild populations. Cross-species amplification was also conducted in five pleuronectiforme species. Of 45 randomly selected and sequenced clones, 43 contained a CA or GA repeat motif. Fourteen pairs of primers were designed to investigate the polymorphism and genetic structure of a wild population collected from North Carolina State coastal waters. Two loci were monomorphic and 12 loci were polymorphic. The number of alleles per polymorphic locus ranged from 2 to 16, with an average of 7.3, and the expected heterozygosity per locus ranged from 0.10 to 0.92, with an average of 0.58. Cross-species amplification showed that most of the markers could successfully amplify Paralichthys olivaceits DNAs, few markers amplified in Verasper variegatus and Verasper moseri, and none of them could amplify Scophthatmus maximus and Cynoglossus semiloevis DNAs. The isolated polymorphic markers would be useful for the genetic breeding and assessment of genetic variation within the genus Paralichthys.

引用

页码：6767 / 6772

页数：6

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