Development of a sensitive method for directly sequencing GII.4 norovirus genome

被引：5

作者：

Xue, Liang

Cai, Weicheng

Wu, Qingping ^{[1
]}

Kou, Xiaoxia

Zhang, Jumei

Guo, Weipeng

机构：

[1] Guangdong Inst Microbiol, State Key Lab Appl Microbiol Southern China, Guangzhou 510070, Guangdong, Peoples R China

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2016年 / 84卷 / 03期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Norovirus; GII.4; Genome; Sequencing; Rational design; Phylogenetic analysis; SYDNEY; 2012; GASTROENTERITIS; RECOMBINATION; PRIMERS; SPREAD; STRAIN; CHINA; PCR;

D O I：

10.1016/j.diagmicrobio.2015.10.022

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A new strategy was proposed for amplifying and sequencing GII.4 norovirus genomes directly. A set of primer pairs was rationally designed, which amplified 6 overlapping fragments encompassing the whole genome. The sensitivity of new primers was comparable to that of detection primers, and 10 viral genome sequences were successfully obtained. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：200 / 202

页数：3

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