Enzyme mass-transfer coefficient in aqueous two-phase systems using static mixer extraction column

被引：4
作者：
Rostami, K [1 ]
Alamshahi, M [1 ]
机构：
[1] Iranian Res Org Sci & Technol, Ctr Biotechnol, Tehran 15819, Iran
来源：
BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2002年 / 25卷 / 03期
关键词：
enzyme mass transfer; static mixer; aqueous two-phase system;
D O I：
10.1007/s00449-002-0292-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Recent technical advances in aqueous two-phase systems (ATPS) have made this a sound technique for the extraction of biomacromolecules. The extraction of alpha-amylase was investigated using aqueous two-phase systems formed by sodium sulphate-polyethylene glycol (PEG) in water in a 47-mm inner diameter spray column packed with three types of static mixers. The effects of dispersed-phase flow rate, phase composition, column height and diameter were studied. The extraction column was operated in a semi-batch manner. It was found that the hold-up and volumetric mass transfer coefficients increased with an increase in dispersed (PEG-rich) phase velocity and decreased with increasing phase composition. Empirical correlations were developed for fractional dispersed-phase hold-up and volumetric mass transfer coefficients.
引用
收藏
页码：169 / 178
页数：10
相关论文
共 29 条
  • [1] Albertsson P. -<Angstrom>., 1985, PARTITION CELL PARTI
  • [2] AlTaweel AM, 1996, CHEM ENG RES DES, V74, P445
  • [3] AMYLASES, ALPHA AND BETA
    BERNFELD, P
    [J]. METHODS IN ENZYMOLOGY, 1955, 1 : 149 - 158
  • [4] BOR TP, 1971, BRIT CHEM ENGNG, P610
  • [5] Calderbank P.H, 1967, CHEM ENG-NEW YORK, V45, P209, DOI 10.1021/cen-v045n045.p052
  • [6] Cybulski A., 1986, Int. Chem. Eng., V26, P171
  • [7] EHART RA, 1976, CHEMIE TECHNIK, V5, P467
  • [8] EHART RA, 1994, BIOTECHNOLOGY, V12, P1113
  • [9] Geankoplis C.J., 1983, TRANSPORT PROCESSES
  • [10] HUSTEDT H, 1985, PARTITIONING AQUEOUS
123