Development of an electrochemical immunosensor for ochratoxin A

被引：45

作者：

Alarcón, SH

Micheli, L

Palleschi, G

Compagnone, D

机构：

[1] Univ Teramo, Dipartimento Sci Alimenti, I-64023 Mosciano Sant Angelo, Teramo, Italy

[2] Univ Roma Tor Vergata, Dipartimento Sci & Tecnol Chim, Rome, Italy

[3] Univ Nacl Rosario, Dept Quim Analit, Fac Ciencias Bioquim & Farmaceut, RA-2000 Rosario, Santa Fe, Argentina

来源：

ANALYTICAL LETTERS | 2004年 / 37卷 / 08期

关键词：

immunosensors; ELISA; ochratoxin A; screen printed electrodes; wine;

D O I：

10.1081/AL-120037586

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A direct, competitive electrochemical enzyme-linked immunosorbent assay (ELISA) has been developed for the quantitative determination of ochratoxin A (OTA) using polyclonal antibodies. The assay is carried out on carbon-based screen printed electrodes (SPE). Optimisation of the ELISA competitive conditions allowed us to realise an assay with improved analytical behaviour compared to the classical spectrophotometric ELISA based assay. The performance was comparable to a published monoclonal based assay. The assay gave a detection limit of 180 pg mL(-1) and sensitivity of 6.1 +/- 0.1 ng mL(-1). The immunosensor was challenged with wine to assess a matrix effect. Recoveries obtained were in the 70-118% range. The method appears to be suitable for OTA contamination screening in food samples.

引用

页码：1545 / 1558

页数：14

共 24 条

[21] 3,3′,5,5′-tetramethylbenzidine as electrochemical substrate for horseradish peroxidase based enzyme immunoassays.: A comparative study [J].